Production of Synthetic Seed from Encapsulated Nodal Segments of Giloe (Tinospora cordifolia Willd.)| Chapter 11 | Research Developments in Science and Technology Vol. 2

Tinospora cordifolia (Cordifolia Tinospora) (Willd.) Miers, also known as giloe, is a plant that belongs to the Menispermaceae family and is used for medicinal purposes, mainly in Ayurveda to treat various ailments.

Standardization of encapsulation matrix and hardening solution concentrations impacting the frequency of germination of Tinospora cordifolia synthetic seeds recorded by efficient plantlet regeneration

Plant Tissue Culture & Genetic Transformation Laboratory, Department of Plant Molecular Biology & Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India, conducted the current study from 2017 to 2020.

Encapsulation of nodal segments of Tinospora cordifolia removed from 18-month-old in vitro generated plants for short-term conservation and future propagation was acknowledged as a method. For a steady supply to meet ever-increasing needs and sustainable resource exploitation, conservation techniques must be employed. To maximise amalgamation and create homogeneous beads, several concentrations and combinations of the gelling matrix, sodium alginate, and the complexing agent, calcium chloride, were used.

2.5 percent sodium alginate and 100 mM calcium chloride were found to be the optimal gelling composition. Murashiage and Skoog's medium, supplemented with 2.0 mgl-1 BAP and 0.2 mgl-1 NAA, yielded the highest conversion rate of encapsulated beads. Hardened, acclimatised, and established regenerants were hardened, acclimatised, and established in net house conditions before being monitored in the field.

The process for producing syn seeds in giloe (Tinospora cordifolia) was standardised in this study. Through artificial seed production, the current technology offers a viable tactic for multiplication, conservation, and germplasm exchange.

Author(s) Details:

Sonali Singh,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.

M. K. Tripathi,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.


Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.

Niraj Tripathi,
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, M. P., India.

G. Tejovathi,
VISM Group of Studies, Gwalior, 474002 M.P., India.

Ashok Ahuja,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, M.P., India.

Please see the link here: https://stm.bookpi.org/RDST-V2/article/view/6697