The goal of the research was to create a simple and sensitive bioanalytical technique for detecting Repaglinide in rabbit plasma for pharmacokinetic studies. The technique was developed utilising a C18 (2) analytical column (5, 2504.6 mm) and a mobile phase of Acetonitrile:0.05 percent Trifluroacetic acid in water (55:45, v/v) at a flow rate of 1 mL/min in RP-HPLC. With suitable system suitability conditions, the retention times of Repaglinide and Rabeprazole were determined to be 4.3 and 5.1 minutes, respectively. The method is linear (r2=0.9987) for a concentration range of 10 to 1000 ng/mL. The accuracy (98.17 percent), intra-, and inter-day precision (2.9 percent), recovery (101.212.19%), and process efficiency (99.773.74%) were all good, with no matrix influence. In bench-top, short-term, and long-term stability analyses, the analyte in samples was shown to be stable for up to 6 hours, 3 freeze-thaw cycles, and not more than 2 months. The pharmacokinetic parameters (Cmax-139.33 ng/mL; Tmax-0.763 h; KE-0.540 h-1; Ka-2.548 h-1; T1/2-1.22 h; AUC0--378.1 ng*h/mL; AUMC0--874.67 ng*h2/mL; MRT-2.32 h; Vd-18.69 L; CLT-10.6 L/h) were determined from time-dependent plasma concentration For the pharmacokinetic evaluation of Repaglinide in rabbit, the established bioanalytical approach proved selective and sensitive.
Raja Navamanisubramanian,
Crescent School of Pharmacy, BSACIST, Vandalur, Chennai, India.
Sabitha Panchagiri,
Department of Pharmacy Practice, Sri Ramachandra Faculty of Pharmacy, SRIHER, Chennai, India.
Raghunandan Nerella,
Department of Pharmaceutical Chemistry, Talla Padmavathi College of Pharmacy, Warangal, India.
Shanmuganathan Seetharaman,
Department of Pharmaceutics, Sri Ramachandra Faculty of Pharmacy, SRIHER, Chennai, India.
Please see the link here: https://stm.bookpi.org/CAPRV-3/article/view/6942
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