The paper presents a genetic method that combined single-cell mRNA differential display and RNA subtractive hybridization 10 years ago to uncover CD8 T-cell quiet. We utilised one comparable RNA specimen from the amplified RNA to execute RNA high-throughput transcriptome sequencing to investigate the viability of whole RNA sequencing technology (RNA-seq) for gene expression at a single-cell level (RNA-Seq). We discovered that RNA-seq results were more closely connected to single-cell mRNA differential display and single-cell qPCR results. The pair correlation of gene expression levels from normalised RPKM (reads per kilobase of transcript per million mapped reads) and qrtPCR among up-regulated genes (Tob, TGF-, LKLF, SnoA, Ski, Myc, ERF, and REST/NRSF complex) in the study was 0.58-0.81. T-cells are in a state of dormancy. The findings show that RNA-Seq may be used to analyse the transcriptome at the single-cell level.
Author(S) Details
Biaoru Li
Department of Pediatrics, Georgia Cancer Center, MCG, Augusta, GA 30912, USA.
Yunbo Xu
Department of Pediatrics, Georgia Cancer Center, MCG, Augusta, GA 30912, USA.
View Book:- https://stm.bookpi.org/NHMMR-V10/article/view/6967
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