Single-cell Whole RNA Sequencing from Tumor-Infiltrating Lymphocyte | Chapter 15 | New Horizons in Medicine and Medical Research Vol. 10

 The paper presents a genetic method that combined single-cell mRNA differential display and RNA subtractive hybridization 10 years ago to uncover CD8 T-cell quiet. We utilised one comparable RNA specimen from the amplified RNA to execute RNA high-throughput transcriptome sequencing to investigate the viability of whole RNA sequencing technology (RNA-seq) for gene expression at a single-cell level (RNA-Seq). We discovered that RNA-seq results were more closely connected to single-cell mRNA differential display and single-cell qPCR results. The pair correlation of gene expression levels from normalised RPKM (reads per kilobase of transcript per million mapped reads) and qrtPCR among up-regulated genes (Tob, TGF-, LKLF, SnoA, Ski, Myc, ERF, and REST/NRSF complex) in the study was 0.58-0.81. T-cells are in a state of dormancy. The findings show that RNA-Seq may be used to analyse the transcriptome at the single-cell level.

Author(S) Details

Biaoru Li
Department of Pediatrics, Georgia Cancer Center, MCG, Augusta, GA 30912, USA.

Yunbo Xu
Department of Pediatrics, Georgia Cancer Center, MCG, Augusta, GA 30912, USA.

View Book:- https://stm.bookpi.org/NHMMR-V10/article/view/6967

A Rare Case Report of Acute T-cell Lymphoblastic Leukaemia Presenting with Cutaneous Involvement in a Child | Chapter 5 | New Frontiers in Medicine and Medical Research Vol. 1

 T-cell lymphoblastic leukaemia with primary cutaneous involvement is uncommon in children. We describe a 6-year-old girl who was hospitalised to our hospital with several skin lesions. She appeared pale and frail. There was lymphadenopathy all over the body. A complete blood test revealed a white blood cell count of 216,000/mm3. A peripheral blood smear revealed 80% lymphoblasts. Flowcytometry demonstrated that 96 percent of the blastic cells in the bone marrow had the immunophenotype and morphological features of acute lymphoblastic leukaemia (T-ALL). The treatment procedure for ALL BFM -95 remission induction was initiated. After two months of cytotoxic therapy, the skin lesion remained the same. After four months of treatment, her symptoms became more severe, and she died.

Author (s) Details

Lohit Kumar Kalita
Department of Oncology, Gauhati Medical College & Hospital, Guwahati, Assam, India.

Chayanika Kalita
Department of Dermatology, Gauhati Medical College & Hospital, Guwahati, Assam, India.

Pabitra Kumar Gogoi
Department of Clinical Hematology, Gauhati Medical College & Hospital, Guwahati, Assam, India.

Umesh Chandra Sharma
Vice-Chancellor, Srimanta Sankadeva University of Health Sciences, Narakasur HillTop, Guwahati, Assam, India.

View Book :- https://stm.bookpi.org/NFMMR-V1/article/view/2784