Single-cell Whole RNA Sequencing from Tumor-Infiltrating Lymphocyte | Chapter 15 | New Horizons in Medicine and Medical Research Vol. 10

 The paper presents a genetic method that combined single-cell mRNA differential display and RNA subtractive hybridization 10 years ago to uncover CD8 T-cell quiet. We utilised one comparable RNA specimen from the amplified RNA to execute RNA high-throughput transcriptome sequencing to investigate the viability of whole RNA sequencing technology (RNA-seq) for gene expression at a single-cell level (RNA-Seq). We discovered that RNA-seq results were more closely connected to single-cell mRNA differential display and single-cell qPCR results. The pair correlation of gene expression levels from normalised RPKM (reads per kilobase of transcript per million mapped reads) and qrtPCR among up-regulated genes (Tob, TGF-, LKLF, SnoA, Ski, Myc, ERF, and REST/NRSF complex) in the study was 0.58-0.81. T-cells are in a state of dormancy. The findings show that RNA-Seq may be used to analyse the transcriptome at the single-cell level.

Author(S) Details

Biaoru Li
Department of Pediatrics, Georgia Cancer Center, MCG, Augusta, GA 30912, USA.

Yunbo Xu
Department of Pediatrics, Georgia Cancer Center, MCG, Augusta, GA 30912, USA.

View Book:- https://stm.bookpi.org/NHMMR-V10/article/view/6967

Determination of Early T-cell Precursor Acute Lymphoblastic Leukemia- A Neoplasm with Dual Lineage Phenotype | Chapter 20 | Recent Developments in Medicine and Medical Research Vol. 10

 ETP-ALL is a subtype of T-ALL/LBL formed from thymic cells in the early T-cell precursor (ETP) development stage, which have the ability to specialise into several lineages, including lymphoid and myeloid. ETP-ALL is responsible for 15% of paediatric T-ALL and 10% to 30% of adult T-ALL. CD7 immunophenotypic expression, a lack of CD1a and CD8, weak CD5 expression (with 75% positive blasts), and positive expression of one or more stem cell or myeloid markers, such as CD117, HLADR, CD13, CD33, CD11b, or CD65, are all present. A well-defined gene expression signature, which includes DNMT3A and FAT3 mutations, identifies ETP-ALL. With a brief review of the literature, we present two cases of ETP-ALL.

Author(S) Details

Surabhi
Department of Pathology, All India Institute of Medical Sciences, Patna, Bihar, India.

A. Singh
Department of Pathology, All India Institute of Medical Sciences, Patna, Bihar, India.

J. Singh Nigam
Department of Pathology, All India Institute of Medical Sciences, Patna, Bihar, India.

View Book:- https://stm.bookpi.org/RDMMR-V10/article/view/4642

Immunity in Medically Important Parasitic Infections: An Overview | Chapter 9 | Recent Progress in Microbiology and Biotechnology Vol. 7

 The rule is immunity. It's frequently unfinished, takes a long time to develop, and then fades away rapidly. The term "immunity" refers to the ability to resist infection. Human life is a battleground in which we are surrounded on all sides by bacteria, viruses, fungus, and parasites, as if we were troops. Our bodies are equipped with an immune system as a defence mechanism. Infections with parasitic worms, such as intestinal worms, are frequently accompanied by blood eosinophilia, which is caused by an inflammatory mechanism. Intestinal infections with Ancylostoma duodenale, Ascaris lumbricoides, Trichuristrichiura, Wuchereria bancroft, Brugiamalayi, loaloa, Dracunculus medinensis, mite infection of the lungs (including at least some cases of tropical eosinophilia), and hydrated disease caused by Echinococcus granulosus are all common causes of blood eos Eosinophilia infiltrates tissues where an antigen-antibody response has occurred in significant numbers. They appear to be drawn to some antigen-antibody reaction product, as evidenced by the fact that when tissues from sensitised guinea pigs mixed with antigen in vitro, or tissues from guinea pigs who have died from anaphylaxis, are transplanted into the peritoneal cavity of normal guinea pigs, the recipient develops severe eosinophilia within 24 hours. Although the active agent has not been infected, it is unlikely that it is histamine. Rodent eosinophils are particularly active phagocytic, ingesting cellular debris, mast cell granules, and other detritus, but it's unclear whether this is true of eosinophils from other species, or what role eosinophils play in these reactions. In parasite infection, a variety of defence systems are activated. When parasites enter the bloodstream (Malaria, Trypanosoma), a humoral response emerges, but parasites that grow within the tissues elicit cell-mediated immunity (Eg: Cutaneous leishmaniasis). B cells, memory cells, and antibodies all play a role in humoral immunity. IgG and IgM are produced in protozoal infections. In addition, IgA is created during an infection of the intestine. IgG, IgM, and IgE antibodies are generated in response to helminthic infections.

Author (S) Details

M. V. R. Rao
Research Laboratory, Apollo institute of Medical Sciences and Research, India.

M. Khaleel
Department of Microbiology, Owaisi Hospital & Research Canter, Deccan College of Medical Sciences, India.

V. K. Chennamchetty
Department of Pulmonology, Apollo Institute of Medical Sciences and Research, India.

S. Rao
Department of Biotechnology, Acharya Nagarjuna University, India.

A. Khan
American University School of Medicine Aruba, Caribbean Islands, India.

T. Calvo
American University School of Medicine Aruba, Caribbean Islands, India.

A. M. Nisanth
NRI Medical College, Dr. NTR University Health Sciences, India.

R. Kudari
Department of Pharmaceutical Analysis, Hindu College of Pharmacy, India.

Dilip Mathai
Department of Medicine, Dean, Apollo Institute of Medical Sciences and Research, India.

M. K. Verma
American University School of Medicine Aruba, Caribbean Islands, India.

View Book :- https://stm.bookpi.org/RPMB-V7/article/view/2579