Plant Regeneration in Rauvolfia serpentina (L.) Benth via Organogenic Mode | Chapter 8| Research Aspects in Biological Science Vol. 7

 In the current study, a successful procedure for Rauvolfia serpentina rapid propagation has been standardised. On MS media that had been treated with various auxins and cytokinins in varying concentrations as well as in various amalgamations, leaf discs and nodal segments were inoculated. to identify the most responsive explants, determine the optimal concentrations of plant growth regulators, and determine other culture conditions that reveal in vitro morphogenesis tracked by plantlet regeneration at higher frequencies through the culture of nodal segments and leaf discs of locally appropriate cultivars. For nodal segment culture, 2.0 mgl-1 of 2, 4-D/NAA alone or in combination with 0.5 mgl-1 TDZ was found to be significantly better for callus initiation than basal MS medium. On inoculation media renewed with TDZ in the range of 0.2-0.5 mgl-1 as solely as well as in association with 0.5 mgl-1NAA, higher shoot proliferating competence, numbers of shoots per explant, and maximum length were observed, though (more than 92% nodal segments initiated shoots, >10.5 shoots/explant, and mean shoot length>3.85 cm). On culture media supplemented with 2.0 mgl-1 auxins: 2,4-D and/or NAA in combination with 0.5 mgl-1 of a cytokinin BA and/or TDZ, however, maximum callus and morphogenic callus formation as well as plantlet regeneration were studied for inoculated leaf discs (more than 76% of leaf discs initiated morphogenic calli with >80% regeneration frequencies). A greater in vitro rooting response was seen in rooting media MS treated with 0.1 mgl-1IBA (root proliferating efficiency, number of roots with higher length).

Author(s) Details:

M. K. Tripathi,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India

G. Tiwari,
Department of Medicinal & Aromatic Plants, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Niraj Tripathi,
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.          

Yashi Singh Tomar,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

D. S. Uikey,
Department of Medicinal & Aromatic Plants, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.

Sharad Tiwari
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

Please see the link here: https://stm.bookpi.org/RABS-V7/article/view/8047

Production of Synthetic Seed from Encapsulated Nodal Segments of Giloe (Tinospora cordifolia Willd.)| Chapter 11 | Research Developments in Science and Technology Vol. 2

Tinospora cordifolia (Cordifolia Tinospora) (Willd.) Miers, also known as giloe, is a plant that belongs to the Menispermaceae family and is used for medicinal purposes, mainly in Ayurveda to treat various ailments.

Standardization of encapsulation matrix and hardening solution concentrations impacting the frequency of germination of Tinospora cordifolia synthetic seeds recorded by efficient plantlet regeneration

Plant Tissue Culture & Genetic Transformation Laboratory, Department of Plant Molecular Biology & Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India, conducted the current study from 2017 to 2020.

Encapsulation of nodal segments of Tinospora cordifolia removed from 18-month-old in vitro generated plants for short-term conservation and future propagation was acknowledged as a method. For a steady supply to meet ever-increasing needs and sustainable resource exploitation, conservation techniques must be employed. To maximise amalgamation and create homogeneous beads, several concentrations and combinations of the gelling matrix, sodium alginate, and the complexing agent, calcium chloride, were used.

2.5 percent sodium alginate and 100 mM calcium chloride were found to be the optimal gelling composition. Murashiage and Skoog's medium, supplemented with 2.0 mgl-1 BAP and 0.2 mgl-1 NAA, yielded the highest conversion rate of encapsulated beads. Hardened, acclimatised, and established regenerants were hardened, acclimatised, and established in net house conditions before being monitored in the field.

The process for producing syn seeds in giloe (Tinospora cordifolia) was standardised in this study. Through artificial seed production, the current technology offers a viable tactic for multiplication, conservation, and germplasm exchange.

Author(s) Details:

Sonali Singh,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.

M. K. Tripathi,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.


Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.

Niraj Tripathi,
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, M. P., India.

G. Tejovathi,
VISM Group of Studies, Gwalior, 474002 M.P., India.

Ashok Ahuja,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002, M.P., India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, M.P., India.

Please see the link here: https://stm.bookpi.org/RDST-V2/article/view/6697

Plantlet Regeneration from Cultured Nodal Segments in Sandalwood (Santalum album Linn.)| Chapter 1 | Research Developments in Science and Technology Vol. 2

By cultured nodal segment, an attempt has been made to compute the appropriate quantity of plant growth regulators to be included in culture media, as well as other physical parameters displaying greater in vitro morphogenesis with 'elite' lines of sandalwood.

On MS medium supplemented with a moderate quantity of TDZ (1.0 mgl-1) in conjunction with a relatively lower concentration of NAA, a higher percentage of direct somatic embryogenesis, number(s) of somatic embryo per explant, and plantlet regeneration by direct organogenesis were observed (0.5 mgl-1). In combination with a lower concentration of NAA (0.5 mgl-1), a greater concentration of BAP (1.0-2.0 mgl-1) enhanced the frequency of indirect somatic embryogenesis. The proportion of organ development directly from the surface of cultured explants was retrieved from culture medium fortified with a higher concentration of BA at 4.0 mgl-1 in conjunction with a lower quantity of NAA (0.5 mgl-1). Plantlets regenerated most efficiently via somatic embryogenesis (direct and/or indirect) on regeneration medium fortified with 2.0 mgl-1TDZ in combination with 1.0 mgl-1GA3, while plantlets regenerated more frequently via indirect organogenesis on regeneration medium fortified with 1.0 mgl-1TDZ in combination with 0.5 mgl-1 GA3 and 0.5 mgl-1 NAA. During the initial weaning phase, the plantlets were moved to pots and hardened in the Environmental Growth Cabinet and Net House before being relocated to the field area. Plants with typical morphology were obtained.

 

Author(s) Details:

M. K. Tripathi,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India.

D. Bele,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India.

Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India.

Nishi Mishra,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India and Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Niraj Tripathi,
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

G. Tiwari,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India and Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Please see the link here: https://stm.bookpi.org/RDST-V2/article/view/6603

Plant Regeneration in Indian Mustard [Brassica juncea (Linn.) Czern & Coss]: Experimental Investigation | Chapter 9 | Current Topics in Agricultural Sciences Vol. 3

 Brassica juncea is an important mustard species that is farmed in India for edible oil. Using mature cotyledons and seeds as explants and embryogenic cell suspension cultures obtained from embryogenic friable calli, the current study establishes an efficient and repeatable plant regeneration process from callus. The best callus induction was achieved using MS basal media enhanced with 3.0 mgl-1 2, 4-D. Supplementing with 0.5 mgl-1 BAP and 0.5-1.0 mgl-1 2,4-D resulted in more regeneration via embryogenesis/organogenesis. It has been established that ripe seeds are preferable as explants, meaning that this explant has greater morphogenic potential. In addition, the genotype, type (s), relative concentrations, and combinations of plant growth regulators all had a significant impact on plantlet regeneration success. These strategies could be utilised in the future to change Indian mustard elite genotypes and select them in vitro.

Author(S) Details

N. Mishra
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

M. K. Tripathi
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

N. Tripathi
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

S. Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

N. Gupta
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

A. Sharma
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

M. K. Shrivastav
Department of Plant Breeding & Genetics, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

View Book:- https://stm.bookpi.org/CTAS-V3/article/view/4630