Plantlet Regeneration from Cultured Nodal Segments in Sandalwood (Santalum album Linn.)| Chapter 1 | Research Developments in Science and Technology Vol. 2

By cultured nodal segment, an attempt has been made to compute the appropriate quantity of plant growth regulators to be included in culture media, as well as other physical parameters displaying greater in vitro morphogenesis with 'elite' lines of sandalwood.

On MS medium supplemented with a moderate quantity of TDZ (1.0 mgl-1) in conjunction with a relatively lower concentration of NAA, a higher percentage of direct somatic embryogenesis, number(s) of somatic embryo per explant, and plantlet regeneration by direct organogenesis were observed (0.5 mgl-1). In combination with a lower concentration of NAA (0.5 mgl-1), a greater concentration of BAP (1.0-2.0 mgl-1) enhanced the frequency of indirect somatic embryogenesis. The proportion of organ development directly from the surface of cultured explants was retrieved from culture medium fortified with a higher concentration of BA at 4.0 mgl-1 in conjunction with a lower quantity of NAA (0.5 mgl-1). Plantlets regenerated most efficiently via somatic embryogenesis (direct and/or indirect) on regeneration medium fortified with 2.0 mgl-1TDZ in combination with 1.0 mgl-1GA3, while plantlets regenerated more frequently via indirect organogenesis on regeneration medium fortified with 1.0 mgl-1TDZ in combination with 0.5 mgl-1 GA3 and 0.5 mgl-1 NAA. During the initial weaning phase, the plantlets were moved to pots and hardened in the Environmental Growth Cabinet and Net House before being relocated to the field area. Plants with typical morphology were obtained.

 

Author(s) Details:

M. K. Tripathi,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India.

D. Bele,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India.

Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India.

Nishi Mishra,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India and Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Niraj Tripathi,
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

G. Tiwari,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India and Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

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