Massive In vitro Propagation from Cultured Nodal Segment of Three Citrus species| Chapter 7 | Research Aspects in Biological Science Vol. 6

 Nodal segments extracted from in vitro developed saplings of three citrus species viz., Citrus aurantiafolia, Citrus reticulata and Citrus sinensis were vaccinated on assorted alterations of basal MS mode for complex shootlet creation. In vitro morphogenesis followed by plant recovery dotted altogether among species and culture medium fortress. In this examination, shootlets were grown straightforwardly by means of helper bud multiplication alongside from callus tissue. Culture medium MS5N.5B/MS5N.Kn (MS + 5.0 mgl-1 NAA + 0.5 mgl-1BA/Kn) persuaded callusing in higher rates. Supplement medium MS.1Td.5N/MS.5B.5N (MS + 0.1 mgl-1 TDZ/0.5 BA + 0.5 mgl-1 NAA) helped plantlet duplicating skill. Though, plantlets per explant in higher frequencies (s) of greater length were perceived on supplement medium MS.1Td or MS.2Td (MS + 0.1/0.2 mgl-1 TDZ). In regard to in vitro establishing, root starting adequacy in higher frequencies was checked on medium MS.5IB (MS + 0.5 mgl-1 IBA), while establishes in more noteworthy numbers were archived on establishing medium MS2IB.5Kn (MS + 2.0 mg l-1 IBA + 0.5 mg l-1 Kn), though supplement medium MS.5IB.5B (MS + 0.5 mg l-1 IBA + 0. 5 mg l-1 BAP) further developed mean root length. In regard to interspecific in vitro reaction, typically, Acid lime followed by Mandarin and Sweet orange performed legitimately for the nearly culture stages. The in vitro created shootlets were effectively adjusted and moved under field conditions.

Author(s) Details:

Megha Vibhute,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.

M. K. Tripathi,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior - 474002, India.

R. Tiwari,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.

Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior - 474002, India.

Niraj Tripathi,
Jawaharlal Nehru Agricultural University, Jabalpur - 482004, India.

Mohini Sharma,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior - 474002, India.

Yashi Singh Tomar,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior - 474002, India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur - 482004, India.

Please see the link here: https://stm.bookpi.org/RABS-V6/article/view/7913

Plantlet Regeneration from Cultured Nodal Segments in Sandalwood (Santalum album Linn.)| Chapter 1 | Research Developments in Science and Technology Vol. 2

By cultured nodal segment, an attempt has been made to compute the appropriate quantity of plant growth regulators to be included in culture media, as well as other physical parameters displaying greater in vitro morphogenesis with 'elite' lines of sandalwood.

On MS medium supplemented with a moderate quantity of TDZ (1.0 mgl-1) in conjunction with a relatively lower concentration of NAA, a higher percentage of direct somatic embryogenesis, number(s) of somatic embryo per explant, and plantlet regeneration by direct organogenesis were observed (0.5 mgl-1). In combination with a lower concentration of NAA (0.5 mgl-1), a greater concentration of BAP (1.0-2.0 mgl-1) enhanced the frequency of indirect somatic embryogenesis. The proportion of organ development directly from the surface of cultured explants was retrieved from culture medium fortified with a higher concentration of BA at 4.0 mgl-1 in conjunction with a lower quantity of NAA (0.5 mgl-1). Plantlets regenerated most efficiently via somatic embryogenesis (direct and/or indirect) on regeneration medium fortified with 2.0 mgl-1TDZ in combination with 1.0 mgl-1GA3, while plantlets regenerated more frequently via indirect organogenesis on regeneration medium fortified with 1.0 mgl-1TDZ in combination with 0.5 mgl-1 GA3 and 0.5 mgl-1 NAA. During the initial weaning phase, the plantlets were moved to pots and hardened in the Environmental Growth Cabinet and Net House before being relocated to the field area. Plants with typical morphology were obtained.

 

Author(s) Details:

M. K. Tripathi,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India.

D. Bele,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India.

Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India.

Nishi Mishra,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV Agricultural University, Gwalior, 474002 M.P., India and Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Niraj Tripathi,
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

G. Tiwari,
Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, Mandsaur – 458001, RVS Agricultural University, Gwalior, M.P., India and Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.

Please see the link here: https://stm.bookpi.org/RDST-V2/article/view/6603