Recent Advancements on Callus and Cell Suspension Cultures: An Effectual Reserve for the Production of Pharmaceutically Significant Metabolites | Chapter 9 | Current Aspects in Pharmaceutical Research and Development Vol. 6

 Alkaloids, glycosides, flavonoids, volatile oils, tannins, resins, and other phytopharmaceuticals are examples of secondary metabolites of pharmaceutical significance. Because their chemical synthesis is either extremely difficult or economically impractical, the majority of these secondary metabolites are currently isolated from wild or cultivated plants. Although production by callus and cell suspension cultures is a lovely alternative, it has only had limited commercial success because to a lack of understanding of how these metabolites are formed. The current book chapter summarises some of the current progress and investigations carried out by authors for the production of useful bioactive secondary metabolites such as withanolides, bacosides, glychyrhizin, reserpine, ajmalicine, amarogentin, plumbagin, and terpenoids from callus and cell suspension cultures of corresponding plant species. Similarly, a lot of thought has gone into it.

Author(S) Details

Ashok Ahuja
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Manoj Kumar Tripathi
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Sushma Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Niraj Tripathi
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

Gyanendra Tiwari
Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

Nishi Mishra
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Shashank Bhargav
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Sharad Tiwari
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

View Book:- https://stm.bookpi.org/CAPRD-V6/article/view/5161

Plant Regeneration in Indian Mustard [Brassica juncea (Linn.) Czern & Coss]: Experimental Investigation | Chapter 9 | Current Topics in Agricultural Sciences Vol. 3

 Brassica juncea is an important mustard species that is farmed in India for edible oil. Using mature cotyledons and seeds as explants and embryogenic cell suspension cultures obtained from embryogenic friable calli, the current study establishes an efficient and repeatable plant regeneration process from callus. The best callus induction was achieved using MS basal media enhanced with 3.0 mgl-1 2, 4-D. Supplementing with 0.5 mgl-1 BAP and 0.5-1.0 mgl-1 2,4-D resulted in more regeneration via embryogenesis/organogenesis. It has been established that ripe seeds are preferable as explants, meaning that this explant has greater morphogenic potential. In addition, the genotype, type (s), relative concentrations, and combinations of plant growth regulators all had a significant impact on plantlet regeneration success. These strategies could be utilised in the future to change Indian mustard elite genotypes and select them in vitro.

Author(S) Details

N. Mishra
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

M. K. Tripathi
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

N. Tripathi
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

S. Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

N. Gupta
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

A. Sharma
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

M. K. Shrivastav
Department of Plant Breeding & Genetics, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

View Book:- https://stm.bookpi.org/CTAS-V3/article/view/4630

Coryphantha macromeris Cell Suspension Cultures: Phytochemical Profiling and Agitation Velocity Effect on Cell Morphology and Viability | Chapter 10 | Recent Research Advances in Biology Vol. 8

 For the generation of active plant metabolites, cell suspension cultures are a viable option. The agitation velocity is one of the most important factors in achieving high cell viability and metabolite yields. The effect of agitation velocity on cell viability and morphology of Coryphantha macromeris cell suspension cultures was investigated in this study, and the phytochemical profile of two-month-old cells cultivated at 80 rpm was analysed using Ultra-High-Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). The results showed that agitation at 120 rpm had a deleterious impact on cell integrity, but at 80 and 100 rpm, the cells survived and proliferated successfully with similar viability percentages (ca. 97 percent ). The presence of 49 metabolites was discovered using chromatographic and mass spectrum analysis, with 45 of them being recognised. Several classes of metabolites were discovered, including phenolic acids (gallic acid derivatives), iridoids (gardoside), stilbenes (tyrolobibenzyl E), lignans (acanthoside B), flavonoids (catechin, lantanoside, sakuranin, afrormosin, kaempferol 7-rhamnoside), and phenylethanoids (phlomisethanoside).

Author (s) Details

E. Cabañas-García
Unidad Profesional Interdisciplinaria de Ingeniería Campus Zacatecas - Instituto Politécnico Nacional. Blvd. del Bote 202 Cerro del Gato, Ejido La Escondida, Col. Ciudad Administrativa, C.P. 98160, Zacatecas, México.

C. Areche
Laboratorio de Productos Naturales Extremos, Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile.

J. Bórquez-Ramírez
Natural Products Laboratory. Chemistry Department. University of Antofagasta. Avenida Universidad de Chile # 028000. C.P. 1240000, Antofagasta, Chile.

R. Muñoz-Miranda
Natural Products Laboratory. Chemistry Department. University of Antofagasta. Avenida Universidad de Chile # 028000. C.P. 1240000, Antofagasta, Chile.

K. M. Rosales-Lopez
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México.

E. Pérez-Molphe Balch
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México.

Y. A. Gómez-Aguirre
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México and CONACyT Research Fellow-Universidad Autónoma de Aguascalientes - Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México.

F. Cruz-Sosa
Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Av. San Rafael Atlixco 186, Col. Vicentina C.P. 09340, Ciudad de México, México.

View Book :- https://stm.bookpi.org/RRAB-V8/article/view/1205