Study on Standardized Rosemary Extract Induce Apoptotic Events, Enhances the Anticancer Effects in Breast Cancer | Chapter 16 | Issues and Developments in Medicine and Medical Research Vol. 9

 Rosemary (Rosmarinus officinalis) is a well-known medicinal herb native to the Mediterranean. Rosemary extracts are a rich source of secondary metabolites, which are commonly employed in food and medicine.

The major goal of this study was to look into the anticancer properties of standardised rosemary extract in human aggressive breast cancer cells and see how it affected apoptosis.

Materials and Methods: Standardized rosemary extract was tested for cytotoxicity using the MTT assay, while acridine orange/propidium iodide dyes were used to assess the apoptotic effects of standardised extracts in breast cancer cells using fluorescence microscopy. The apoptotic impact of standardised rosemary extract was then assessed using flow cytometry and western blotting.

Results: Standardized rosemary extracts had a strong cytotoxic effect on MCF-7 cells, with IC50 values of 50.75g/mL. Fluorescence microscopy and Annexin V-propidium iodide double-staining revealed that standardised rosemary extract potentiates apoptosis. Western blotting results, on the other hand, revealed upregulation of proapoptotic proteins, activation of PARP-1, Caspase 9, and a concentration-dependent decrease in the expression of the anti-apoptotic protein Bcl-2, as well as an increase in the expression level of the proapoptotic protein BAX.

In conclusion, standardised rosemary extract inhibited cell proliferation and induced apoptosis in breast cancer cells. As a result, it has the potential to be developed as an anticancer treatment to slow the progression of cancer; however, more research is needed.

Author(S) Details

K. Gouthamchandra
Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore-560105, Karnataka, India.

H. V. Sudeep
Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore-560105, Karnataka, India.

H. B. Lingaraju
Department of Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore-560105, Karnataka, India.

K. Shyam Prasad
Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore-560105, Karnataka, India and Department of Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore-560105, Karnataka, India.

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