Monday, 28 February 2022

Culture of Cyclops for Use the First Intermediate Host in Experimental Life Cycle of Spirometra Species: An Experimental Research | Chapter 12 | Issues and Developments in Medicine and Medical Research Vol. 9

 Background: Under normal circumstances In the life cycle of Spirometra species, cyclops are the first intermediate hosts.

The purpose of this research is to describe how Cyclops were cultivated in a Tanzanian laboratory from an adult female egg sack Cyclops fed live Paramecium and a stock of Cyclops employed in a Spirometra species life cycle experiment.

Paramecium was first cultured for use as a food source for Cyclops. A sample of water was taken from a pond on the campus of Sokoine University. A swarm of Paramecium was created when 100 mL of water and pre-boiled wheat grains were transferred to a Petri dish and incubated under laboratory conditions for 7 days. In a new Petri dish containing tap water, 0.3 cc of Paramecium suspension, and 4 pre-boiled wheat grains, an adult female egg sack Cyclops from a natural water pond in Tarangire National Park, Tanzania was introduced. The mixture was stored at a temperature of 26-29°C in a laboratory and monitored on a daily basis.

Eggs laid by a single Cyclops hatched into nauplius. The average development time from nauplius I to copepodite I was 1.2 days, copepodite I to adult female Cyclops was 6.9 days, and copepodite I to adult female Cyclops was 26.3 days. The adult male was 846.3 metres long and 284.6 metres wide, whereas naupliusI was 120.2 metres long and 80.0 metres wide. 1 to 8 broods were generated by the mature female (mean 4.3). Cyclops lived for an average of 43.1 days.

Conclusion: In the experimental life cycle of Tanzanian Spirometra species, laboratory-cultured Cyclops were fed Paramecium and served as the first intermediate host.

Author(S) Details

Nicholas Jairo Kavana
St. Francis University College of Health and Allied Sciences, P.O Box 175, Ifakara, Tanzania.

View Book:- https://stm.bookpi.org/IDMMR-V9/article/view/5847

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