The goal of this study was to see how different surface treatments affected zirconia's osteoblastic activity. The osseointegration concept is intimately related to the success of endosseous implants. The topography of a biomaterial's surface has a significant impact on osseointegration. A total of 60 Zirconia disc samples were manufactured and divided into three groups. Group 1 [n-20]: Zirconia without treatment; Group 2 [n-20]: Zirconia treatment with sandblasting/acidic etching; Group 3 [n-20]: Zirconia treatment with UV light. Following the surface treatment, the surface roughness and topography of all three groups were measured using a SEM scanning electron microscope. Human osteoblastic sarcoma cells were seeded at a density of 1 X 104 cells per cm2 on the test material and cultured for 48 hours at 37 + 10c in a humidified environment containing 5% CO. Cell seeded test material and glass cover slips were treated with 2.5 percent glutaraldehyde after 48 hours. The adherence of osteoblastic cells and colony formation on the zirconia discs were evaluated by SEM and compared between the groups. When comparing zirconia discs that had been treated with UV radiation, etching, and sand blasting to zirconia discs that had not been treated, there was a significant increase in osteoblast adhesion. Group I samples demonstrated reduced osteoblastic cell spread and fewer osteoblastic cell colonies due to cell adhesion. The osteoblastic cells in Group II samples were more evenly distributed than those in Group I. They had less osteoblastic cell colonies, though. In comparison to Group I and Group II, the cell adhesion exhibited in Group III (UV Photofunctionalisation) revealed more noticeable osteoblast cells that were dispersed throughout the Sample and showed superior adhesion. Animal research should be conducted in the future. Because this is an in vitro study, it cannot be fully translated to in vivo circumstances. The tissue reaction under in vivo settings must be assessed.
Author(S) Details
Roseline Meshramkar
Department of Prosthodontics, SDM College of Dental Sciences, Dharwad, India.
Praveen Kumar Shetty
Department of Biochemistry, SDM College of Medical Sciences, Dharwad, India.
Gouri V. Anehosur
Department of Prosthodontics, SDM College of Dental Sciences, Dharwad, India.
Aishwarya Nayak
Department of Prosthodontics, SDM College of Dental Sciences, Dharwad, India.
K. P. Lekha
Department of Prosthodontics, SDM College of Dental Sciences, Dharwad, India.
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