Standardization of In-vitro Regeneration Protocol in Gerbera jamesonii Bolus Ex Hooker F.| Chapter 7 | Current Topics in Agricultural Sciences Vol. 7

The gerbera, which belongs to the Asteraceae family, is the most popular cut flower and is in the top 10 cut flowers in the world. Planting material on a huge scale is necessary for commercial propagation of this plant species, which necessitates the use of plant tissue culture techniques for vast in vitro replication.

By growing mature embryo in vitro, an attempt was made to compute the ideal dose of plant growth regulators provided in culture medium and optimise other physical parameters demonstrating higher in vitro response.

Results showed that the nutrient medium MS3D.5B (MS + 3.0 mgl-1 2, 4 D + 0.5 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar powder) was better for callus initiation. MS2N.5iP/MS3N.5ip (MS + 2.0/3.0mg l-1 NAA + 0.5 mgl-12-ip + 30.0 gl-1 sucrose + 7.5 gl-1 agar) elicited a greater in vitro response, as measured by the number of shoot proliferating explants and shoot (s) per explant. On the other hand, shoots of greater length were recovered on the MSB/MS2B culture medium (MS + 2.0/3.0 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar). On the rooting medium MS.1IB (MS + 0.1 mgl-1IBA + 15.0 gl-1 sucrose + 7.5 g l-1 agar), there was an improved in vitro rooting response (root proliferating efficiency, number of roots, and root length). The regenerants were potted and hardened in the Environmental Growth Cabinet and net House before being successfully transplanted to field settings. Normal phenotypic plants were obtained.

Author(s) Details:

Deepa Bhatt,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.

M. K. Tripathi,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

M. Vidhya Sankar,
Department of Floriculture and Landscaping, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.

Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Mohini Sharma,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.

Niraj Tripathi,
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

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