Investigation of Hypolipidemic Activity of Nanoparticles Containing Ethanolic Extract of Cocculus pendulus in High Fat Fed Rats | Chapter 2 | Challenges and Advances in Pharmaceutical Research Vol. 2

Cardiovascular disease is caused by an abnormal presence of lipids in the circulation (CVD). The major goal of this work was to make nanoparticles from an ethanolic extract of Cocculus pendulus and test their hypolipidemic efficacy in rats fed a high-fat diet. Preliminary phytochemical examination of the complete plant of Cocculus pendulus indicated the presence of alkaloids, phytosterols, flavonoids, triterpenes, and volatile oils, among other phytoconstituents. HPTLC fingerprinting of an ethanolic extract of the plant Cocculus pendulus displays 13 peaks at 256 nm and 10 peaks at 366 nm, respectively, while GC-MS analysis demonstrates the presence of -Sitosterol as a marker component. The use of nanotechnology to improve the bioavailability of herbal medicines is a promising strategy. The optimal nanoformulation revealed a mean particle size, polydispersity index, and zeta potential of 536 nm, 0.256, and 16.4 mV, respectively, of ethanolic extract of Cocculus pendulus nanoparticles prepared using the Solvent evaporation approach. On male Wistar rats, antiatherogenic efficacy was tested using ethanolic extract dosages of 200, 400 mg/kg and optimised nanoparticles doses of 40, 80 mg/kg. The effects of two different dosages of ethanolic extract of Cocculus pendulus and nano formulations on an 8-week high-fat diet were investigated. Finally, results show that when Cocculus pendulus and Nano formulations were administered orally at two dose levels, there was a significant reduction in body weight, total cholesterol, low density lipoprotein, phospholipids, triglycerides, and VLDL levels, as well as a similarly significant increase in high density lipoprotein levels. Cocculus pendulus nanoparticles with doses of 40 mg/kg and 80 mg/kg had better hypolipidemic action than the ethanolic extract of Cocculus pendulus with doses of 200 mg/kg and 400 mg/kg.

Author(s) Details:

Sampath Kumar Ramala,
Department of Pharmacy, Annamalai University, Annamalainagar-608002, Tamil Nadu, India.

G. Alagumanivasagam,
Department of Pharmacy, Annamalai University, Annamalainagar-608002, Tamil Nadu, India.

A. Kottai Muthu,
Department of Pharmacy, Annamalai University, Annamalainagar-608002, Tamil Nadu, India.

Please see the link here: https://stm.bookpi.org/CAPR-V2/article/view/6574