In vitro Studies on Proliferation and Production of Cytokine and IgG by Human PBMCs Stimulated with Polysaccharide Extract from Plants Endemic to Gabon | Chapter 8 | Current Advances in Chemistry and Biochemistry Vol. 4

 Polysaccharides were collected from seven Gabonese plants to investigate their immunological capacity. Proliferation, cytokine, and immunoglobulin G (IgG) assays in peripheral blood mononuclear cells (PBMCs) (5 105 cells/mL) were performed after stimulation with different concentrations of polysaccharide fractions, compared to lipopolysaccharides (LPS) and concanavalin A (ConA) from healthy volunteers. The culture supernatants were used in an enzyme-linked immunosorbent assay to detect cytokines and IgG. (ELISA). Pectin and hemicellulose extracts from Uvaria klainei, Petersianthus macrocarpus, Trichoscypha addonii, and Aphanocalyx microphyllus were found to be effective. For IL-12 (3–40 pg/mL), IL-10 (6–443 pg/mL), IL-6 (7–370 pg/mL), GM-CSF (3–170 pg/mL), and IFN- (5–80 pg/mL), Librevillea klaineana, Neochevalierodendron stephanii, and Scorodophloeus zenkeri induced output levels that were variable from one Only Aphanocalyx microphyllus hemicelluloses contain a small amount of IgG (OD = 0.034), but cells stimulated with these polysaccharides proliferate up to 318 percent faster than cells that are not stimulated. When the pectin of some of these plants was treated with endopolygalacturonase, however, the proliferation of PBMCs was stopped (p 0.05). However, both before and after enzymatic treatment or saponification, the pattern of cytokine synthesis remained the same. These polysaccharides appear to stimulate cells in a structure-dependent manner, according to this research. The rhamnogalacturonan-I (RGI) fragment did not induce PBMC proliferation on its own. Polysaccharides' immunostimulatory properties are confirmed in this review.

Author (s) Details

Line Edwige Mengome
Institutde Pharmacopée et de MédecineTraditionnelles (IPHAMETRA), BP 1935 Libreville, Gabon and Laboratoire Glyco-MEV, IRIB, Université de Rouen, 76821 Mont-Saint-Aignan, France.

Aline Voxeur
Laboratoire Glyco-MEV, IRIB, Université de Rouen, 76821 Mont-Saint-Aignan, France.

Jean Paul Akue
Centre International de Recherches Médicales de Franceville (CIRMF), BP 769 Franceville, Gabon.

Patrice Lerouge
Laboratoire Glyco-MEV, IRIB, Université de Rouen, 76821 Mont-Saint-Aignan, France.

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