Monday, 31 May 2021

Development of RP-HPLC Method for Simultaneous Estimation of Metformin and Pioglitazone in Pharmaceutical Formulation | Chapter 11 | Technological Innovation in Pharmaceutical Research Vol. 3

A fast, selective, speedy, accurate, and cost-effective reverse phase HPLC technique has been devised for the simultaneous quantification of metformin and pioglitazone from pharmaceutical dosage forms. The experiment was carried out on a phenomenex C18 column (25 cm x 4.6 mm i.d., 5 mm i.d., 5 mm i.d., 5 mm i.d., 5 mm i.d., 5 mm i.d., 5 mm i.d., phosphate buffer phase containing acetonitrile (adjusted to pH 5.0 using orthophosphoric acid) at a flow rate of 1. ml/min (50:50 v/v). The wavelength of detection was 258 nm. As a measure of internal control, The drug etoricoxib was utilized. The retention times for paracetamol, aceclofenac, and etoricoxib were 4.75, 6.44, and 6.44, respectively. 8.83 minutes and 8.83 minutes, respectively. In terms of precision, linearity, detection limit, and detection limit, The established method was validated in terms of quantitation and solution stability. These medications' dosage forms in combination dosage forms can be calculated using the provided procedure. Raw materials, formulations, and dissolution tests can all be checked using the current RPHPLC equipment.

Author (s) Details

S. Alexandar
Department of Pharmaceutical Chemistry, Vinayaka Mission’s College of Pharmacy, Vinayaka Missions Research Foundation (Deemed to be University), Yercaud Main Road, Salem – 636008, India.

View Book :- https://stm.bookpi.org/TIPR-V3/article/view/1154

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