A reverse phase HPLC method for simultaneous measurement of Atrovastatin and Ezetimibe from pharmaceutical dosage forms has been developed. It is simple, selective, quick, exact, and cost-effective. At a flow rate of 1 ml/min, the procedure was performed on a Phenomenex C18 (25 cm x 4.6 mm i.d., 5 ) column with a mobile phase of Water and 0.4 percent (v/v) TEA: Acetonitrile (adjusted to pH 6.5 using Orthophosphoric acid) (60:40 v/v). At a wavelength of 248 nm, the detection was performed. Pioglitazone was utilized as an internal control. Retention times for atrovastatin, ezetimibe, and pioglitazone were 3.42, 6.90, and 4.28 minutes, respectively. The established method's accuracy, precision, linearity, limit of detection, limit of quantitation, and solution stability have all been validated. The proposed approach can be used to estimate these medications' dose forms in combination dose forms. Raw materials, formulations, and dissolution tests can all be checked using the current RP-HPLC technology.
Author (s) DetailsS. Alexandar
Department of Pharmaceutical chemistry, Vinayaka Mission’s College of Pharmacy, Vinayaka Missions Research Foundation (Deemed to be University),Yercaud Main Road, Salem – 636008, India.
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