A Cytomorphometric Analysis of Oral Mucosal Changes in Tobacco Users | Chapter 15 | Highlights on Medicine and Medical Research Vol. 10

 The goal of this study was to use cytomorphometry to look at the effects of tobacco chewing and smoking on the buccal mucosa.

Methodology & Materials: 45 subjects were divided into three groups: smokers, chewers, and nonsmokers, with oral mucosal cells collected from each. The cells were stained with Papanicolaou stain and viewed under a microscope at a high magnification.

To calculate the Cellular Diameter, Nuclear Diameter, and Nuclear Cytoplasmic Ratio, Image J analysis software was used to perform cytomorphometric analyses on the cells in the acquired image. ANOVA was employed as the statistical test, and p0.05 was considered significant.

Results: Cellular diameter declines as smokers and chewers progress, whereas nuclear diameter and nuclear cytoplasmic ratio increase.

Conclusion: Chewing tobacco modified the cytomorphology of normal buccal mucosa, and the degree of alteration was greater in chewers than in smokers, according to the data.

Oral smears can be cytomorphometrically analysed on a regular basis to detect changes in cellular and nuclear dimensions, especially in tobacco users.

Author(s) Details

Anju Mathew
Department of Oral Medicine and Radiology, Pushpagiri College of Dental Sciences, Perumthuruthy, Thiruvalla, Kerala, India.

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