AgNOR as a Proliferative Marker in the Diagnosis of Oral Cancer | Chapter 7 | Novel Aspects on Pharmaceutical Research Vol. 3

 Tumor evaluating and typing has an important part in evalution and assessing the administration of tumor both clinically and too the prognosis. Even though histopathological disease is golden standard, but some periods the pathology picture forsake to differentiate between mild or malignant tumors. To overcome this deficit, the study was designed to use buccal smears for diagnosis that is less cost effective, well allowed by the patients and maybe repeated some number of times. So the present study proposed at evaluating the proliferative index of the spoken epithelial cells taken from a buccal besmirch in three different groups that is to say normal subjects, compartment for smokers and tobacco chewers utilizing AgNOR staining.

Author(s) Details:

S. Prasanna,
Department of Oral Pathology and Microbiology, Seema Dental College and Hospital, Rishikesh, Uttarakhand, India.

N. Srikant,
Department of Oral Pathology, Manipal College of Dental Sciences, Mangalore, India.

Please see the link here: https://stm.bookpi.org/NAPR-V3/article/view/10882

Bio-Monitoring and Occupational Risk Assessment in Human Population from Nuclear Research Centre - using Exfoliated Buccal Epithelial Cells and Peripheral Blood Lymphocytes | Chapter 6 | Current Overview on Disease and Health Research Vol. 2

 

The goal of the study is to evaluate the baseline frequency of micronuclei in exfoliated buccal epithelial cells (BE cells) and peripheral blood lymphocytes from the same people in a randomly selected human population of a nuclear research facility. A non-invasive method to assess radiation and mutagen exposure is urgently needed. Our nuclear research center's monitoring personnel evaluated the buccal epithelial micronuclei test for this reason when they were there for their yearly physicals. The micronucleus test is a sensitive indicator that counts the number of broken or lost chromosomes. To assess the baseline micronuclei frequency in exfoliated buccal epithelial cells and lymphocyte micronuclei, a study was conducted on 50 randomly selected persons working in nuclear research centres. test for buccal micronucleus cytome On slides stained with Geimsa's dye, the blood lymphocyte cytokinesis blocked micronuclei assay (CBMN) and BMNcyt were carried out. We provide the average frequency of spontaneous micronuclei in blood lymphocytes and buccal epithelial cells. The average number of micronuclei (MN) found in buccal epithelial cells of men and females was 0.49 and 0.10, respectively, with a range of 0.09 to 2.92. (range 0.00 to 2.06). MN frequency was 0.66 0.10 in buccal epithelial cells (range 0.00 to 2.92). The MN frequencies were determined to be 8.61 0.10 (range 3.00 to 17.00) in females and 9.15 1.00 (range 3.00 to 22.00) in men in human peripheral blood cells from the same donors. In cytokinesis-blocked lymphocytes, the overall MN frequency was 8.96 0.73 Bi-nucleated (BN) cells (Range: 3.00 to 22.00). Human peripheral blood lymphocytes and buccal epithelial cells both have spontaneous micronuclei at a baseline frequency that is correlated. As a result, buccal epithelial cell micronuclei provide for an effective noninvasive biomonitoring technique.

Author(s) Details:

N. K. Sharma,
Low Level Radiation Research Section, Radiation Biology and Health Sciences Division, Biosciences Group, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India

S. D. Kamble,
Pathology Laboratory, Occupational Health Centre, Medical Division, Modular Laboratory, Bhabha Atomic Research Centre, Trombay Mumbai 400085, India.

D. Jeyaprakash,
Occupational Health Centre, Medical Division, Modular Laboratory, Bhabha Atomic Research Centre, Trombay Mumbai 400085, India.

 

Please see the link here: https://stm.bookpi.org/CODHR-V2/article/view/7746

Biopsy – As a Diagnostic Tool | Chapter 16 | Recent Developments in Medicine and Medical Research Vol. 15

 A biopsy is the removal of tissue from a living organism for the purposes of diagnosis and therapy. Appropriate training and good hand skills are required for a successful biopsy procedure. Different biopsy procedures exist, each with its own set of advantages and disadvantages. As a result, in order to achieve a definitive diagnosis, the dental practitioner must choose the appropriate biopsy procedure.

Author(S) Details

K. Nitya
Department of Oral and Maxillofacial Pathology, Adhiparasakthi Dental College & Hospital, Melmaruvathur, Tamil Nadu, India.

V. Keerthinarayan
Department of Oral Pathology, Thai Moogambikai Dental College, Tamil Nadu, India.

B. Rajprakash
Department of Oral and Maxillofacial Surgery, Adhiparasakthi Dental College & Hospital, Melmaruvathur, Tamil Nadu, India.

K. T. Shanmugam
Department of Oral and Maxillofacial Pathology, Best Dental College and Hospital, Madurai, India.

M. Dinesh Dhamodhar
Department of Public Health Dentistry, SRM Dental College, Ramapuram, Chennai, India.

Raj Mohan
Department of Public Health Dentistry, SRM Dental College, Ramapuram, Chennai, India.

M. Karthikeyan
Dept of Dentistry, Shri Sathya Sai Medical College and Research Institute, India.

Nancy Solomon
Department of Pediatric and Preventive Dentistry, SRM Dental College, Ramapuram, India.

View Book:- https://stm.bookpi.org/RDMMR-V15/article/view/5018

A Cytomorphometric Analysis of Oral Mucosal Changes in Tobacco Users | Chapter 15 | Highlights on Medicine and Medical Research Vol. 10

 The goal of this study was to use cytomorphometry to look at the effects of tobacco chewing and smoking on the buccal mucosa.

Methodology & Materials: 45 subjects were divided into three groups: smokers, chewers, and nonsmokers, with oral mucosal cells collected from each. The cells were stained with Papanicolaou stain and viewed under a microscope at a high magnification.

To calculate the Cellular Diameter, Nuclear Diameter, and Nuclear Cytoplasmic Ratio, Image J analysis software was used to perform cytomorphometric analyses on the cells in the acquired image. ANOVA was employed as the statistical test, and p0.05 was considered significant.

Results: Cellular diameter declines as smokers and chewers progress, whereas nuclear diameter and nuclear cytoplasmic ratio increase.

Conclusion: Chewing tobacco modified the cytomorphology of normal buccal mucosa, and the degree of alteration was greater in chewers than in smokers, according to the data.

Oral smears can be cytomorphometrically analysed on a regular basis to detect changes in cellular and nuclear dimensions, especially in tobacco users.

Author(s) Details

Anju Mathew
Department of Oral Medicine and Radiology, Pushpagiri College of Dental Sciences, Perumthuruthy, Thiruvalla, Kerala, India.

View Book :- https://stm.bookpi.org/HMMR-V10/article/view/1126