The goal of this work was to employ high-performance thin layer chromatography (HPTLC) to detect glycosides from petroleum ether and methanol extracts of medicinally and commercially beneficial leaves of Holoptelea integrifolia (Roxb.) planch.
Materials and Methods: HPTLC investigations were performed after preliminary phytochemical screening. Linomat V applicator is included with the Camag HPTLC system (Switzerland).
With the help of a tungstant lamp,
densitometric scanning was done with a Camag TLC scanner IV in the reflectance
absorbance mode at 540 nm using Win computer-aided transcribing system software
(1.4.6 Camag).
Results: Steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins,
tannins, and carbohydrates were found in preliminary phytochemical screening of
H. integrifolia petroleum extract, while steroids, alkaloids, flavonoids,
proteins, and carbohydrates were found in preliminary phytochemical screening
of H. integrifolia methanolic extract. HPTLC fingerprinting of glycosides from
a leaf extract in petroleum ether revealed four polyvalent phytoconstituents (4
peaks) with escalating retention factor (Rf) values in the range of 0.37-0.82.
whereas a methanol extract of the leaves revealed ten Polyvalent
phytoconstituents (10 peaks) with Rf values in the range of 0.20-0.83 in
ascending order.
Author (s) Details
Ravindra C. Sutar
Department of Pharmacology, Sanjivani College of Pharmaceutical Education and Research, Kopargaon - 423 603, Ahmednagar, Maharashtra, India.
View Book :- https://stm.bookpi.org/TIPR-V3/article/view/1147
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