Recent Development and Validation of Omeprazole Analysis Methods in Capsules with Absorbance Methods and Areas under Curves Methods with UVVis Spectrophotometry | Chapter 5 | Technological Innovation in Pharmaceutical Research Vol. 3

Two simple spectrophotometric methods have been devised to analyze omeprazole in capsules. Sodium hydroxide 0.1 N is employed as a solvent in this procedure. At 304.80 nm, the absorbance method was utilized, and at wavelengths ranging from 281.60 nm to 333.60 nm, the under-curve area method was utilized. Over a concentration range of 10 g / mL to 18 g / mL, both techniques were determined to be linear. The correlation coefficient for the absorbance method is 0.9998, whereas the area-under-curve approach has a correlation coefficient of 0.997. 105.48 percent of generic was obtained using the absorbance technique. The field under the curve approach yielded 102.87 percent with omeprazole pills. The absorbance method yields a percentage of the trademarked omeprazole capsules of 104.02 percent. The percentage obtained using the field under the curve method was 103.62 percent. The proportion of both The percentage of samples that match the criteria of Pharmacopoeia Indonesia version V, which ranges from 90% to 110%, is 90%. to a maximum of 110 percent The relative standard deviation for both techniques is 2%. According to statistical analysis, the absorbance approach and the region under the curve did not differ significantly (sig. 2-tailed> 0.05). As a result, the absorbance method and the region under the curve of UV-Vis spectrophotometry can be used to determine omeprazole levels in formulation capsules.

Author (s) Details

Harrizul Rivai
Faculty of Pharmacy, Andalas University, Limau Manih Campus, Padang 25163, Indonesia.

Rizqa Hasanah
School of Pharmaceutical Science, Jl. Tamansiswa No. 9, Padang 25138, Indonesia.

Zikra Azizah
School of Pharmaceutical Science, Jl. Tamansiswa No. 9, Padang 25138, Indonesia.

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