The goal of this study is to see if patients with inflammatory bowel diseases (IBDs) are resistant to routine treatment. Immunosuppressive medications like sirolimus (SIR) and tacrolimus (TAC) have been proven to work. However, they have adverse effects that limit their use. Metformin (MET) is an anti-diabetic drug. medication, having anti-inflammatory properties that are promising. As a result, the goal of this research was to confirm the effect of the In the treatment of experimentally produced pain, concurrent administration of MET with SIR or TAC colitis is a disease that affects the digestive system. The Dextran sulphate (DSS) induced colitis model was used in this study.
Methodology: For 9 days, colitis was induced by giving 5 percent DSS in water twice a day via oral gavage. MET 200 mg/kg can be used alone or in conjunction with SIR. On day 7, 1 mg/kg or TAC 1 mg/kg was begun and continued for a total of 12 days. After that, histological and immunohistochemical staining of distal colon tissues was performed. The levels of pro-inflammatory cytokines such as tumour necrosis factor-alpha (TNF-), interleukin (IL)-1, IL-6, and IL-17A were then evaluated in tissue homogenates. The effects of DSS and the levels of all proinflammatory cytokines were dramatically reduced by MET, SIR, or TAC. Furthermore, MET enhances the effects of SIR and TAC.
Conclusion: When it came to DSS-induced colitis, MET exhibited a significant anti-inflammatory effect. As a result, it could be a viable adjuvant therapy for IBD management. Inhibition of NF-B activation was one of the mechanisms behind the impact. This study can be utilized as a foundation for pilot clinical trials to examine the effect of MET on IBD, given the size of its efficacy and its safety profile. The findings of this study, however, need to be further confirmed and translated into clinical consequences.Author (s) Details
Rania M. Magadmi
Pharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Fahad H. Aljahdali
Pharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia and Compliance Management at Directorate of Health Affairs in Jeddah City, Jeddah, Saudi Arabia.
Mustafa Alsawy
Department of Histology and Cytology, Faculty of Medicine, Al-Azhar University, Egypt.
Ahmed S. Ali
Pharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia and Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Egypt.
Fatemah O. Kamel
Pharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
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