The goal of this study was to discover DNA variation among planted Brassica juncea accessions using a set of SSR molecular markers. Genetic diversity estimate is critical not only for crop development programmes, but also for the effective management and conservation of existing genetic variability. Using multiple morphological characteristics such as days to 50% blooming and maturity, genetic diversity was examined in forty germplasm lines and eight cultivars of Indian mustard. plant height (cm), length of main raceme (cm), number(s) of primary and secondary branches/plants, number(s) of siliques per plant and seeds per silique, 1000 seed weight (g), seed yield per plant (g), and 50 SSR molecular markers in preliminary investigation, of which 7 SSR molecular markers were found to be highly polymorphic between all mustard genotypes and were used in advance research. For each of the 48 genotypes, a UPGMA phonogram was created, with similarity coefficients ranging from 0.00 to 0.91. The number of alleles ranged from 3 to 4, genetic diversity was between 71 and 65 percent with a mean of 67 percent, heterozygosity was between 10 and 20% with an average of 12 percent, and PIC values for markers were between 0.65 and 0.59 percent with a mean of 0.61 percent. The PIC value of all seven SSR primers was more than 0.5 (50 percent), indicating considerable genetic diversity in the genetic stock tested.
Narendra Singh Rajpoot,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
M. K. Tripathi,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
R. S. Tomar,
Rani Laxmibai Central Agricultural University, Jhansi, India.
Niraj Tripathi,
Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
R. S. Sikarwar,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
S. S. Tomar,
Office of Dean, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Please see the link here: https://stm.bookpi.org/RDST-V4/article/view/6858
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