Introduction: Peanut (Arachis hypogaea L.) is a valuable income crop with several uses for each plant component. Groundnut rust and late leaf spot (LLS) are two major foliar fungal diseases that can reduce yields by up to 70%. Fungicide use is costly and harmful to the environment; thus, developing new cultivars with genetic resistance is a more sustainable, environmentally friendly, and cost-effective way.
The goal of this study was to use allele specific molecular markers to screen
groundnut germplasm lines for foliar fungal infections (LLS and rust).
The current study included 30 uncharacterized germplasm lines and four peanut
check variants. Groundnut germplasm lines were screened against foliar fungal
diseases such as LLS and rust using a set of four gene-based SSR molecular
markers.
Results: A total of 14 alleles were found for polymorphic SSR markers, with an
average of 3.5 alleles per locus. The gene diversity and Polymorphic
Information Content (PIC) values varied from 0.3972-0.5778, with an average of
0.47. The genetic relationships between peanut genotypes are represented in a
UPGMA tree based on SSRs. Based on origin, four primary demographic groupings
were constructed using principal coordinate analysis (PCA). STRUCTURE v2.3.3
software based on SSR markers was used to examine the population structure of
the 34 genotypes. Structure Harvester was used to find the optimum K value, and
the largest peak was found at delta K = 2. Based on maximum likelihood and
delta K (dK) values, a total of K subpopulations (K) were discovered, with two
core and pure groups and an admixture group. AH8054, CS21181, CS708, and Akola
White were discovered as four germplasm lines that might be exploited as foliar
disease resistant cultivars for groundnut hybridization and improvement.
Punam Chand Bhawar,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
M. K. Tripathi,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
R. S. Sikarwar,
Department of Genetics & Plant Breeding, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M. P, India.
R. S. Tomar,
Rani Laxmibai Central Agricultural University, Jhansi, India.
Niraj Tripathi,
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Please see the link here: https://stm.bookpi.org/RDST-V4/article/view/6860
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