Nowadays, it is vital to value chicken feather agro-waste; if these wastes are discarded without treatment, they may contribute to environmental difficulties. Increased chicken production will result in a greater release of feather waste into the environment. Traditional chicken feather processing methods such as land filling, chemical treatment, and burning are costly, time-consuming, and environmentally dangerous. Microbial hydrolysis, on the other hand, is now often recognised as the most environmentally friendly recycling method. As a result, the purpose of this study is to identify keratinolytic bacteria capable of effective feather hydrolysis, as well as to optimise key physical parameters that may influence bacterial development and, as a result, degrading capability. Bacillus sp., a newly discovered bacteria, was discovered to have outstanding feather-destroying abilities. D4 strain isolated from chicken feathers at 37°C, pH 8.0, and 106CFU/mL cell size inoculum, with greatest keratin activity (55.01.35 U/mL) and total protein content in the cell free supernatant of 0.65mg/mL, respectively. Apart from the monetary values of these variables, At 40°C (35.12 U/mL), 25°C (30.12 U/mL), and pH 7.5 (52.32 U/mL), pH 9.0 (49.01.2 U/mL), and pH 10.0 (38.2 1.35 U/mL), there was a moderate amount of enzyme activity. Bacillus sp. D4, on the other hand, could not handle high alkaline pH values of 11.0, acidic pH values of 4.0 and 5.0, or a high temperature of 55°C, resulting in low enzyme activity (19.0 1U/mL).
Author (s) Details
Dr. Amal A. Al Moussa
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh P.O.Box 145111, ZIP, 4545, Saudi Arabia.
Nadine M. S. Moubayed
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh P.O.Box 145111, ZIP, 4545, Saudi Arabia.
Amani M. Al Jaloud
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh P.O.Box 145111, ZIP, 4545, Saudi Arabia.
Dr. Fatima S. Al Khattaf
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh P.O.Box 145111, ZIP, 4545, Saudi Arabia.
Noura D. Dahmasha
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh P.O.Box 145111, ZIP, 4545, Saudi Arabia.
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