In-vitro Antibacterial Activity of Thymol and Eugenol against Ralstonia solanacearum Isolated from Potato | Chapter 03 | Innovations in Microbiology and Biotechnology Vol. 5

Potato output (Solanum tuberosum L.) is severely hampered by bacterial wilt induced by Ralstonia solanacearum. R. solanacearum is mostly spread by infected seed stocks, although it can also be transferred by agricultural machinery, irrigation water, and plant-to-plant transmission. rhizosphere. There are currently no known conventional bactericides that are effective in controlling this soil-borne pathogen. At this time, there is no pathogen. The goal of this study was to look into the antibacterial properties of eugenol and thymol. against R, which are the main components of thyme and clove essential oils, respectively. solanacearum. The pathogen was found in potato tubers that had developed bacterial wilt signs. Disc To assess the inhibitory properties, diffusion and minimum inhibition concentration (MIC) techniques were used. the effects of these compounds A combinational test was carried out using the chequerboard assay. Thymol At 100 mg mL-1, and eugenol showed 27 mm and 17 mm inhibition zones, respectively. Both The bactericidal effects of eugenol and thymol against R. solanacearum were observed, and their MICs were determined. 275 g mL-1 and 175 g mL-1, respectively, were determined. In a combinational study, an additive impact was discovered. The results of the test showed that using both of them together did not significantly improve inhibition. Both compounds are toxic. have the potential to be employed as antibiotics to treat bacterial wilt disease, albeit thymol has a lower potency than the others. a higher level of concentration than the other However, in vivo use of these volatile compounds is problematic. Encapsulating them in an appropriate nanocarrier can solve this problem.

Author (s) Details

George Oluoch
Institute for Basic Sciences Technology and Innovation, Pan African University, Kenya.


Edward George Mamati
Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology, Kenya.


Viviene Matiru
Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Kenya.


Moses Nyongesa
National Potato Research Centre, Kenya Agricultural and Livestock Research Organization, Kenya.

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