Thermophilic Bacteria have the Toxin-Antitoxin System too: Type II Toxin-Antitoxin System Composites in Geobacillus | Chapter 01 | New Innovations in Chemistry and Biochemistry Vol. 6

 The toxin-antitoxin (TA) systems are important regulators of stress responses in prokaryotes and have biotechnological applications. As a result, they've gotten a lot of research interest. While the type II TA system of mesophiles has garnered a lot of research, thermophiles have gotten a lot less. The potential type II TA families encoded on the genomes of four thermophilic strains of the Geobacillus genus are shown here. We mined for TA-coding genes using the TA finder tool and manually curated the results using protein domain analysis tools. To uncover the geobacilli TA features, we used the NCBI BLAST, Operon Mapper, ProOpDB, and sequence alignment tools. We found 28 putative TA pairs spread among eight TA families. 15 potential new toxins and antitoxins from the MazEF, MNT-HEPN, ParDE, RelBE, and XRE-COG2856 TA families were found among the discovered TAs. AbrB-ParE, a potentially novel TA compound, was also discovered. In addition, we propose the Geobacillus acetyltransferase TA (GacTA) family, which could be one of the few TA families having a reverse gene order. The goal of this study is to highlight the importance of investigating TAs in thermophiles in general and Geobacillus in particular, as well as to facilitate future experimental research.

Author (S) Details

Rawana N. Alkhalili
Department of Biology, Lund University, SE-223 62 Lund, Sweden.

Joel Wallenius
Department of Biology, Lund University, SE-223 62 Lund, Sweden.

Bjorn Canback
Department of Biology, Lund University, SE-223 62 Lund, Sweden.

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