The goal of this work was to determine the experimental conditions under which the cationic polymer poly-L-lysine produces effective and safe desquamation of the urothelium of the urinary bladder. Induced urothelium desquamation is a common method for studying urothelium regeneration as well as creating animal disease models. Chitosan has previously been found to cause urothelial cells to desquamate on a huge scale. We looked at the capacity of another positively charged polymer, poly-L-lysine, to desquamate. Ex vivo and in vivo tests were used to investigate its impact on the urothelium's functional and structural integrity. The results demonstrated that the concentration of poly-L-lysine had the greatest impact on the structure and function of the urothelium, whereas the treatment period and molecular weight of poly-L-lysine had little impact. In ex vivo and in vivo trials, fifteen minutes of urothelium exposure to 0.01 percent poly-L-lysine was found to be effective and safe, resulting in severe cell desquamation that was limited to the superficial cell layer only. Ex vivo, functional and structural regeneration of the urothelium occurred 2 to 3 hours after poly-L-lysine-induced damage. As a result, poly-L-lysine appears to be a promising inducer of urothelial cell desquamation that could be employed in the clinic to treat urologic illnesses.
Author(S) Details
Andreja Erman
University of Ljubljana, Faculty of Medicine, Institute of Cell Biology, Vrazov trg 2, 1000 Ljubljana, Slovenia.
Mojca Kerec Kos
University of Ljubljana, Faculty of Pharmacy, Askerceva Cesta 7, 1000 Ljubljana, Slovenia.
View Book:- https://stm.bookpi.org/CAPRD-V7/article/view/5418
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