Study on the Prevalence of Extended-spectrum B-lactamase in Pseudomonas aeruginosa in the Tertiary Care Center, India | Chapter 11 | Innovations in Microbiology and Biotechnology Vol. 3

 The purpose of this study was to find out how often ESBL production is among clinical isolates of Pseudomonas aeruginosa.

Pseudomonas aeruginosa is an opportunistic nosocomial infection with a rising resistance to broad-spectrum beta-lactams, which is mediated by extended-spectrum beta-lactamase enzymes (ESBL).

Pseudomonas aeruginosa was discovered using normal microbiological protocols after various clinical specimens were processed in our laboratory. All isolates were subjected to an ESBL screening test. Using the Disc Diffusion approach, the suspected ESBL producer was then exposed to an ESBL phenotypic confirmation test. In accordance with Clinical Laboratory Standard Institute (CLSI 2016) guidelines, all confirmed isolates were subjected to an antimicrobial susceptibility test using the Kirby – Bauer disc diffusion method.

During the study period, 322 non-duplicate Pseudomonas aeruginosa isolates were discovered. ESBL production was detected in 26.09 percent of Pseudomonas aeruginosa isolates (n = 84). Ciprofloxacin 79 (94.05 percent), Gentamicin 61 (72.62 percent), and tobramycin 60 resistance was found in all ESBL positive Pseudomonas aeruginosa isolates (71.43 percent ). Resistance to medicines such as cefoperazone + sulbactam 17 (20.24%), piperacillin + Tazobactam 14 (16.67%), and Imipenem 15 was low (17.86 percent ). Polymyxin B sensitivity was 100 percent in all isolates.

Conclusion: The current study highlights the prevalence and drug resistance of Pseudomonas aeruginosa that is ESBL positive. Antibiotic judicious usage necessitates regular antimicrobial susceptibility testing to prevent the spread of drug resistance.

Author(S) Details

Swati Tewari
Department of Microbiology, Mulayam Singh Yadav Medical College and Hospital, Meerut, Uttar Pradesh, India.

Alok Kumar
Department of Microbiology, Mulayam Singh Yadav Medical College and Hospital, Meerut, Uttar Pradesh, India.

Ekta Rani
Department of Microbiology, Mulayam Singh Yadav Medical College and Hospital, Meerut, Uttar Pradesh, India.

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