Isolation and Identification of Non Fermenting Gram Negative Bacilli: A Prospective Study | Chapter 13 | Innovations in Microbiology and Biotechnology Vol. 4

 Non-fermenting Gram-Negative Bacilli (NFGNB) pathogens that have been related to nosocomial infections include Pseudomonas aeruginosa, Acinetobacter baumanii, Burkholderia cepacia complex (BCC), and Stenotrophomonas maltophilia. Previously, NFGNB were thought to be either commensals or pollutants. The purpose of this study was to identify the various nonfermenters isolated from patients admitted to our tertiary care hospital in Jammu. The lab obtained 4585 clinical specimens, including 1234 urine, 742 pus, and 1864 blood cultures collected in a brain-heart infusion broth, 110 endotracheal catheter tips, 529 CSF samples, and 115 body fluids, as well as 110 endotracheal catheter tips, 529 CSF samples, and 115 body fluids. These samples were cultivated on blood agar, chocolate agar, and Mac Conkey's agar for 18-24 hours at 37°C. Established methodologies and traditional biochemical assays were used to identify the clinical isolates. A total of 572 of 4585 clinical specimens were found to have non-fermenting Gram-negative bacilli, resulting in a 12.40 percent isolation rate. Pseudomonas aeruginosa was the most common strain, accounting for 312 (54.54 percent), with Acinetobacter baumanii 235 (41.08 percent), Stenotrophomonas maltophilia 15 (2.62 percent), and Burkholderia cepacia complex 10 following closely behind (1.70 percent). In our investigation, thirteen isolates (86.66 percent) were sensitive to Colistin, while four isolates (26.60 percent) were susceptible to Imipenem. As a result, NFGNB has risen to prominence as a major opportunistic disease resistant to traditional antimicrobials. As a result, early detection and empirical treatment based on the institute's local antibiogram data will reduce mortality and improve patient management.

Author(S) Details

Ruchita Mahajan
Department of Microbiology, GMC, Jammu, India.

. Neeraj
Department of Orthopaedics, GMC, Jammu, India.

Sarika
ASCOMS, Jammu, India.

Bella Mahajan
Department of Microbiology, GMC, Jammu, India.

View Book:- https://stm.bookpi.org/IMB-V4/article/view/6458

Study on the Prevalence of Extended-spectrum B-lactamase in Pseudomonas aeruginosa in the Tertiary Care Center, India | Chapter 11 | Innovations in Microbiology and Biotechnology Vol. 3

 The purpose of this study was to find out how often ESBL production is among clinical isolates of Pseudomonas aeruginosa.

Pseudomonas aeruginosa is an opportunistic nosocomial infection with a rising resistance to broad-spectrum beta-lactams, which is mediated by extended-spectrum beta-lactamase enzymes (ESBL).

Pseudomonas aeruginosa was discovered using normal microbiological protocols after various clinical specimens were processed in our laboratory. All isolates were subjected to an ESBL screening test. Using the Disc Diffusion approach, the suspected ESBL producer was then exposed to an ESBL phenotypic confirmation test. In accordance with Clinical Laboratory Standard Institute (CLSI 2016) guidelines, all confirmed isolates were subjected to an antimicrobial susceptibility test using the Kirby – Bauer disc diffusion method.

During the study period, 322 non-duplicate Pseudomonas aeruginosa isolates were discovered. ESBL production was detected in 26.09 percent of Pseudomonas aeruginosa isolates (n = 84). Ciprofloxacin 79 (94.05 percent), Gentamicin 61 (72.62 percent), and tobramycin 60 resistance was found in all ESBL positive Pseudomonas aeruginosa isolates (71.43 percent ). Resistance to medicines such as cefoperazone + sulbactam 17 (20.24%), piperacillin + Tazobactam 14 (16.67%), and Imipenem 15 was low (17.86 percent ). Polymyxin B sensitivity was 100 percent in all isolates.

Conclusion: The current study highlights the prevalence and drug resistance of Pseudomonas aeruginosa that is ESBL positive. Antibiotic judicious usage necessitates regular antimicrobial susceptibility testing to prevent the spread of drug resistance.

Author(S) Details

Swati Tewari
Department of Microbiology, Mulayam Singh Yadav Medical College and Hospital, Meerut, Uttar Pradesh, India.

Alok Kumar
Department of Microbiology, Mulayam Singh Yadav Medical College and Hospital, Meerut, Uttar Pradesh, India.

Ekta Rani
Department of Microbiology, Mulayam Singh Yadav Medical College and Hospital, Meerut, Uttar Pradesh, India.

View Book:- https://stm.bookpi.org/IMB-V3/article/view/5363