Brucellosis is a zoonosis that is present all over the world and has significant public health and economic ramifications. Brucella abortus and Brucella melitensis are the most common causes. The goal of this study was to assess the sensitivity and specificity of the immunochromatographic assay (ICA) for serodiagnosis of sheep and goat brucellosis, and to compare the results with those obtained from the Rose Bengal Plate Test (RBPT) and direct Enzyme Linked Immunosorbent Assay (ELISA). Antibodies against Brucella were found in 8 (38.09 percent), 6 (28.57 percent), and 16 (76.19 percent) of 41 serum samples (21 rams and 20 male goats) examined by RBPT, (ICA), and iELISA, respectively, in ram samples (8 (38.09 percent), 6 (28.57 percent), and 16 (76.19 percent), and in 3 (15 percent), 1 (5 percent), and 12 (60 percent) of male goats' serum samples respectively. In rams, the kappa value between iELISA and ICA was -0.08 and RBPT and ELISA was -0.01, whereas in male goats, the kappa value between iELISA and ICA was -0.06 and RBPT and ELISA was -0.14. In rams, sensitivity for RBPT and ICA was 37.5 percent and 25%, respectively, while specificity was 60 percent and 60 percent; in male goats, sensitivity for RBPT and ICA was 8.33 percent and 8.33 percent, respectively, while specificity was 75 percent and 100 percent. It can be concluded that the proposed ICA is a reliable immunodiagnostic assay that is quick, low-cost, and cost-effective, making it ideal for large-scale screening in developing nations and rural regions. The goal of this study was to determine the diagnostic value of the ICA device for ovine and caprine brucellosis serodiagnosis and compare the results to those obtained using RBPT and iELISA.
Author(S) Details
Waffa A. Ahmed
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.
Shaimaa A. Majeed
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.
Ameer H. Abdul Ameer
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.
Nawal D. Mahmmod
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.
Nawal D. Mahmmod
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.
Nameer I. Saeed
Ministery of Agriculture, Agriculture Research Office, Baghdad, Iraq.
Luma Y. Hanaa
Vet Research & Laboratories Department, Baghdad, Iraq.
View Book:- https://stm.bookpi.org/RAAVS-V6/article/view/6990
No comments:
Post a Comment