A Rare and Fatal Case of Bilateral, Upper, Lower Limbs and Abdominal Gangrene Linked to Brucellosis | Chapter 1 | Microbiology and Biotechnology Research: An Overview Vol. 1

Brucellosis is one of the most commonly encountered zoonotic diseases predominantly caused by Brucella melitensis, Brucella abortus, and Brucella suis, with an estimated incidence of 5 lakh new cases/year. Brucellosis distributed worldwide is a classical zoonotic disease. Complications associated with gangrene are quite uncommon and may or might not occur during the course of the disease. Here, the case of a pediatric age group patient who presented with fever and gangrene over the upper and lower limbs and abdomen associated with brucellosis was presented. The patient had an antibody titer of 1:1280. Hence, routine serological diagnosis in patients with clinical signs and symptoms suggestive of brucellosis is essential. This also helps in detecting more number of cases. It was concluded that brucellosis still remains a serious threat and poses a severe impact on public health concerns, particularly where the disease is endemic. Hence, a high index of suspicion is really necessary when patients present with unexplained symptoms, especially for those who are at an occupational risk for brucellosis.

 

Author (s) Details

Tejashree Anantha
Department of Microbiology, JSS Medical College and Hospital, JSSAHER, Mysore, Karnataka, India.

 

Vidyavathi B. Chitharagi
Department of Microbiology, JSS Medical College and Hospital, JSSAHER, Mysore, Karnataka, India.

 

M. Krishna Karthik
Department of Microbiology, JSS Medical College and Hospital, JSSAHER, Mysore, Karnataka, India.

 

Jagadeesh Kumar
Departments of Pediatrics, JSS Medical College and Hospital, JSSAHER, Mysore, Karnataka, India.

 

B. Sukesh Kumar
Department of Microbiology, JSS Medical College and Hospital, JSSAHER, Mysore, Karnataka, India.

 

Badveti Satya Sai
Department of Microbiology, JSS Medical College and Hospital, JSSAHER, Mysore, Karnataka, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/mbrao/v1/4416

Determination of Sensitivity and Specificity of Various Serological Tests for Detection of Brucella spp. Infection in Male Goats and Sheep | Chapter 07 | Research Aspects in Agriculture and Veterinary Science Vol. 6

 Brucellosis is a zoonosis that is present all over the world and has significant public health and economic ramifications. Brucella abortus and Brucella melitensis are the most common causes. The goal of this study was to assess the sensitivity and specificity of the immunochromatographic assay (ICA) for serodiagnosis of sheep and goat brucellosis, and to compare the results with those obtained from the Rose Bengal Plate Test (RBPT) and direct Enzyme Linked Immunosorbent Assay (ELISA). Antibodies against Brucella were found in 8 (38.09 percent), 6 (28.57 percent), and 16 (76.19 percent) of 41 serum samples (21 rams and 20 male goats) examined by RBPT, (ICA), and iELISA, respectively, in ram samples (8 (38.09 percent), 6 (28.57 percent), and 16 (76.19 percent), and in 3 (15 percent), 1 (5 percent), and 12 (60 percent) of male goats' serum samples respectively. In rams, the kappa value between iELISA and ICA was -0.08 and RBPT and ELISA was -0.01, whereas in male goats, the kappa value between iELISA and ICA was -0.06 and RBPT and ELISA was -0.14. In rams, sensitivity for RBPT and ICA was 37.5 percent and 25%, respectively, while specificity was 60 percent and 60 percent; in male goats, sensitivity for RBPT and ICA was 8.33 percent and 8.33 percent, respectively, while specificity was 75 percent and 100 percent. It can be concluded that the proposed ICA is a reliable immunodiagnostic assay that is quick, low-cost, and cost-effective, making it ideal for large-scale screening in developing nations and rural regions. The goal of this study was to determine the diagnostic value of the ICA device for ovine and caprine brucellosis serodiagnosis and compare the results to those obtained using RBPT and iELISA.

Author(S) Details

Waffa A. Ahmed
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.

Shaimaa A. Majeed
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.

Ameer H. Abdul Ameer
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.

Nawal D. Mahmmod
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.

Nawal D. Mahmmod
Zoonosis Diseases Unit, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq.

Nameer I. Saeed
Ministery of Agriculture, Agriculture Research Office, Baghdad, Iraq.

Luma Y. Hanaa
Vet Research & Laboratories Department, Baghdad, Iraq.

View Book:- https://stm.bookpi.org/RAAVS-V6/article/view/6990