In the current study, an effort was made to provide a standardised method for the separation and characterisation of enterococci by using Fourier Transform Infrared spectroscopy [FTIRS] to type Enterococcus sp. that was isolated from a hospital. One of the most frequent causes of nosocomial infections, the Enterococcus genus can be challenging to treat due to the emergence of Vancomycin-resistant strains. For epidemiological research, the examination of Enterococcus isolates is essential. Enterococci are typically typed using conventional phenotypic and genotypic methods. FTIR spectroscopy data were compared to results from PCR amplification of the vancomycin gene and tests for antibiotic sensitivity; the study revealed that 6 isolates were positive for the van gene (4 of VanA, 1 of VanB and 1 VanA plus VanB). VanB was shown to be susceptible, but three of VanA and VanA plus VanB were resistant to every antibiotic tested (Ampicillin, Teicoplanin, and Vancomycin). The isolates were classified into 8 groups using FTIR spectroscopy (first derivatives). The remaining 13 Enterococcus isolates were classified into 3 clusters, with one group each of VanA (4 isolates), VanB (1 isolate), and VanA + VanB (1 isolate). In comparison to other approaches, the study showed that FTIR spectroscopy has a good ability for discrimination and a high level of reproducibility. This finding also suggests that certain Enterococci bacterial species produce organic molecules that may be used to classify them, and it is thought that FTIR has a lot of promise for use in this kind of clinical microbiology.
Mohammad I. Abu Taha,
Department of Physics Al-Quds University, Jerusalem, Palestinian Territories.
Hatem K. Eideh,
Department of Medical Laboratory Sciences, Al-Quds University, Jerusalem, Palestinian Territories.
Sameeh M. Saed,
Iskander Khoury Secondary School, Bethelehem, Palestinian Territories.
Hazem Jaber,
Medical Lab, Al-Maqased Hospital, Jerusalem, Palestinian Territories.
Please see the link here: https://stm.bookpi.org/IMB-V6/article/view/7242
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