New Bioengineering Strategies Based on Tissue-Specific Extracellular Matrices to Improve Human Endometrial Organoid Models | Chapter 9 | New Horizons in Medicine and Medical Research Vol. 11

 Multicellular, three-dimensional (3D) tissue models called organoids closely mimic in vivo tissue. Exact pre-clinical models of the human uterine endometrium are being used to conduct new studies of this vital tissue in response to the recent emergence of 3D structures from basic endometrial cells. We aimed to assess ECM hydrogels produced from decellularized pig endometrium (EndoECM) in producing and upgrading current human endometrial organoid culture systems in order to enhance these 3D models. To do this, we developed three lines of human endometrial organoids and expanded them under ideal and unfavourable circumstances while adding or omitting the soluble supplement EndoECM (0.1 mg/mL). The resultant organoids' epithelial genesis, long-term chromosomal stability, and stemness characteristics were all validated. Finally, we calculated their proliferative capacity under various culture conditions using proliferation rates and immunohistochemical methods. Our results show the importance of a bioactive environment for the upkeep and development of human endometrial organoids.

Author(s) Details:

Emilio Frances-Herrero,
Universitat de Valencia, 46010 Valencia, Spain and  Fundacion Instituto Valenciano de Infertilidad (FIVI), Instituto de Investigacion Sanitaria La Fe, 46026 Valencia, Spain.

Elena Juarez-Barber,
Fundacion Instituto Valenciano de Infertilidad (FIVI), Instituto de Investigacion Sanitaria La Fe, 46026 Valencia, Spain.

Hortensia Ferrero,
Fundacion Instituto Valenciano de Infertilidad (FIVI), Instituto de Investigacion Sanitaria La Fe, 46026 Valencia, Spain.

Irene Cervello,
Fundacion Instituto Valenciano de Infertilidad (FIVI), Instituto de Investigacion Sanitaria La Fe, 46026 Valencia, Spain.

Please see the link here: https://stm.bookpi.org/NHMMR-V11/article/view/7144