The adsorption of hen egg white lysozyme (HEWL) on adzuki bean charcoal powder (ABCP), which was made from adzuki bean waste by pyrolysis without combustion under a nitrogen atmosphere and subsequently comminution with a jet mill, significantly improved HEWL's heat stress tolerance. Even at high temperatures, HEWL was well-adsorbed on ABCP. The remaining activity of HEWL adsorbed on ABCP was 30% after 30 minutes of incubation at 90°C, increased with incubation time at 25°C, plateaued at 30 minutes, and became around 50%. On the other hand, native HEWL activity is essentially non-existent after 30 minutes of incubation at 90°C, and it is unaffected by incubation at 25°C. In comparison to the native one, the thermal denaturation curve of HEWL adsorbed on ABCP was pushed to high temperatures. Furthermore, at 90°C, BCP-adsorbed HEWL had a half-life of 28 minutes, whereas native HEWL had a half-life of 4 minutes.
Author (S) Details
Hidetaka Noritomi
Department of Applied Chemistry for Environment, Tokyo Metropolitan University, Minami-Ohsawa, Hachioji, Tokyo 192-0397, Japan.
View Book :- https://stm.bookpi.org/NFMMR-V6/article/view/2761
No comments:
Post a Comment