Bioluminescence is a natural phenomenon found in living
things that emit light without emitting heat.
Bioluminescence can be produced in three ways, namely
from special photocytes cells, glands in
tissues, and luminescent bacteria that are symbiotic to
the host. Bioluminescence in Loligo sp and
Harpadon
nehereus is known to originate from luminescent bacteria which are
symbiotic in
luminescent organs. However, information about
symbiotic bacteria in other types of luminescence
biota and its potential in Indonesian waters has not
been widely explored. This study aims to
determine the potential of luminescence symbiotic
bacteria in Loligo sp. and Loligo edulis
from Jepara
waters and Harpadon nehereus from the
Malacca Strait waters, Indonesia as an anti-bacterial agent
and to prove differences in the luminescence proteins
produced by symbiotic bacteria and their hosts.
Sample collection is done by isolating the bacteria
from luminescent organs which are then used as
research samples. The study was conducted using a
qualitative activity test on pathogenic bacteria
and analysis of SDS-PAGE electrophoresis protein in
bacteria and its host. The study found 2 isolates
of luminescent symbiotic bacteria that were potential
as antibacterial agents against Multi Drug
resistant (MDR) pathogens of Escherichia
coli, with inhibition zones of 32.59 mm and of Enterobacter
sp.5 with inhibition zones of 28.44 mm.
Luminescent symbiotic bacteria of L. edulis have the
potential
to be antibacterial agent against Vibrio
harveyi, E. coli, Staphylococcus aureus, and Bacillus sp.
Analysis of H. nehereus symbiotic
bacteria showed gamma hemolytic in blood agar test. Protein
analysis of SDS-PAGE electrophoresis showed that the
enzymes found insymbionic bacteria were
different from the enzymes in their host.
Author
(s) Details
Prof. Dr. Delianis
Pringgenies,
Department of Marine Sciences,
Faculty of Fishery and Marine Sciences, Diponegoro University, Tembalang
Campus, Semarang, Indonesia.
Kartika Dewi
Department of Marine Sciences, Faculty of Fishery and Marine Sciences,
Diponegoro University, Tembalang Campus, Semarang, Indonesia.
Pramita Apriliyani
Department of Marine Sciences, Faculty of Fishery and Marine Sciences,
Diponegoro University, Tembalang Campus, Semarang, Indonesia.
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