Lectin is a protein of non-immunological origin. It is a
protein with agglutination properties. They have
biological properties that can be exploited for
medicinal and therapeutic purposes. The objective of
this study was to isolate and characterize lectin
activity in Texas Live Oak (Quercus fusiformis). More
specifically, the study aimed to determine the lectin’s
blood group specificity and pH stability,
determine effects of seasonal variation, soil moisture
and soil pH on lectin activity. The study also
aimed to determine the presence of antifungal activity
in Q. fusiformis extracts. Lectin activity was
detected and compared via agglutination and protein
assays. Protein partial purification was
accomplished using diethylaminoethyl ion-exchange
chromatography matrix. High Performance Liquid Chromatography
(HPLC) was used to assess purity of the lectin. Results showed that Q. fusiformis extracts’ lectin activities are stable at a pH range of 5.2 -
9.2 but with a significant decrease in activity above pH 9.2. Lectin activity was
significantly higher when assayed against sheep red blood cells as compared to other blood groups tested. Quercus
fusiformis extract is devoid of antifungal activity against Aspergillus
niger and Rhizopus stolonifer. Seasonal
variation, soil moisture, and soil pH do not significantly correlate with lectin activity. Results
from HPLC showed presence of three peaks indicating
a partial purification of the Q. fusiformis lectin.
Author (s) Details
Ruby A. Ynalvez
Department of Biology and Chemistry, Texas A & M International
University, Laredo, Texas, USA.
Carmen Cruz-Mac Kinnon
Department of Biology and Chemistry, Texas A & M International
University, Laredo, Texas, USA.
Marcus A. Ynalvez
Department of Social Sciences, Texas A & M International University,
Laredo, Texas, USA.
View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/268
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