Aims: To investigate and compare the
protective and ameliorative role of aqueous leaf extract of
Andrographis
paniculata and standard drug 5-Fluorouracil on selected organs of male
Wistar rats
exposed to benzene carcinogen.
Study
Design: Histological assessment of protective and ameliorative
activity of the aqueous leaf
extract of Andrographis paniculata and standard
drug 5-Fluorouracil in some organs of experimental
animals exposed to benzene carcinogen.
Place
and Duration of Study: Department of Biomedical Sciences
Ladoke Akintola University of
Technology, College of Health Sciences, Osogbo. Nigeria
between May and August 2012.
Methodology:
Seventy-two (72) young male Wistar rats weighing 100-120 g were grouped
into six
(A-E), each group comprised of 6 rats in two replicates
of three each (n=12).
Group A (control) received distilled water and normal
saline (0.5 ml/kg each), group B received
benzene chromasolv (0.2 ml at 1:10 dilution
(water/2-propanol), group C received aqueous
Andrographis paniculata (10 mg/kg bodyweight), group D
was administered 5 -fluorouracil only (5
mg/kg body weight) for four weeks, group E was
pretreated with Andrographis paniculata for 4 weeks
prior to the administration of benzene chromasolv and
group F was post treated with Andrographis
paniculata for 4 weeks after pre-exposure to
benzene chromasolv for 8 weeks. Leukaemia burden
was assessed using haematological parameters such as
Packed cell volume, Haemoglobin
concentration, Red blood cells count and Total
leukocyte count in the control and treatment groups.
Results:
Results showed that leukemia was induced within 8 weeks as a
significantly elevated WBC
(leukocyte) counts and anaemia over the group not
exposed to benzene which served as baseline
were noted (p< 0.05).Organ histology showed varying
lesions of the heart (mild to marked) coronary
congestion, severe vacuolar degeneration and necrosis
of the hepatocytes with cellular infiltration by
mononuclear cells, diffuse tubular degeneration and
necrosis with renal interstitial hemorrhage
observed in the groups exposed to benzene carcinogen,
5-Fluorouracil and co-treatments of these
two agents. However, hepato-renal and heart
histio-architectures were intact in the extract pretreatment and
post-treatment
groups.
Conclusion:
These results suggest that Andrographis paniculata might be more
effective than some
other drugs currently in use as cancer suppressive
chemotherapeutic agents and also may be a novel
bioagent for the treatment of acute or chronic injuries
induced on the liver, kidneys and heart by
toxicants and carcinogens.
Author
(s) Details
Akanni E. Olufemi
Department of Biomedical Sciences, College of Health Sciences, Ladoke
Akintola University of Technology, P.M.B 4400, Osogbo, Nigeria.
O. R. Folarin
Department of Biomedical Sciences, College of Health Sciences, Ladoke
Akintola University of Technology, P.M.B 4400, Osogbo, Nigeria.
C. Igbeneghu
Department of Biomedical Sciences, College of Health Sciences, Ladoke
Akintola University of Technology, P.M.B 4400, Osogbo, Nigeria.
View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/268
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