This study was carried out to validate the method of dosage
of Aflatoxin B1 in the groundnut pastes
for high performance liquid chromatography, according
to the NFV03-110 Mai 2010 standard. The
tests of the validation were performed out starting
from ten samples. The linearity of calibration
established between 0 and 3.0 μg/L was proven by
determining the square of the coefficient of
correlation of Pearson (R2 = 0.9985).
The limits of detection and quantification were respectively of
0.01 µg/kg and 0.02 µg/kg. The coefficients of
variation of the tests of repeatability and reproducibility
are respectively 3.4% and 3.79%, lower than 5%. The
rate of recovery were estimated at 107.6%. The test
of conformity did not show a significant difference (P ˃ 0,05) between the
average concentration in aflatoxine B1 (2.02 ±
1.44 μg/kg) and that of the certified value (2 μg/kg). The method of
quantifying aflatoxin B1 in peanut paste by
using HPLC can be regarded as acceptable for an analysis of routine.
Author
(s) Details
Zamblé Bi Irié Abel
Boli
Department of Food Science and Technology (UFR-STA), Laboratory of
Biotechnology and Food Microbiology, University Nangui Abrogoua, 02 BP 801,
Abidjan 02, Côte d’Ivoire.
Fatoumata Camara
Department of Food Science and Technology (UFR-STA), Laboratory of
Nutrition and Food Safety, Université Nangui Abrogoua, 02 BP 801, Abidjan 02,
Côte d’Ivoire.
Djegba Marie Toka
Department of Food Science and Technology (UFR-STA), Laboratory of
Biotechnology and Food Microbiology, University Nangui Abrogoua, 02 BP 801,
Abidjan 02, Côte d’Ivoire.
Marina Koussemon
Department of Food Science and Technology (UFR-STA), Laboratory of
Nutrition and Food Safety, Université Nangui Abrogoua, 02 BP 801, Abidjan 02,
Côte d’Ivoire.
Rose Koffi-Nevry
Department of Food Science and Technology (UFR-STA), Laboratory of
Biotechnology and Food Microbiology, University Nangui Abrogoua, 02 BP 801,
Abidjan 02, Côte d’Ivoire.
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