The first attempt dared on Dalbergia
melanoxylon tissue culture was conducted from 2010 to 2013 in
the Botany Laboratory University of Dar es Salaam. A
total of 500 seeds were sterilized at different
concentration of reagents and inoculated at different
strength of the Murashige and Skoog medium for
germination to obtain disease free explants for callus
induction trials. A total of 400 nodal segments
obtained from germinated seeds were sterilized at
different concentration of reagents and inoculated
at different hormonal combinations to induce callus
formation for seedling multiplication. Results from
this tissue culture attempt set a foundation for tissue
culture success in Dalbergia melanoxylon on the
future research. Only 19.8% of seeds inoculated in half
strength of Murashige and Skoog medium
germinated within 7 days while only 6.8% of seeds
inoculated in full strength germinated within 6
days. A total of 1% of inoculated Dalbergia
melanoxylon fragments in Murashige and Skoog media
supplemented with hormone combination at 2.0 mg/l BAP +
0.5 mg/l NAA developed callus after16
days from the inoculation day. The final weight of the
callus at the last record was 0.62 g. The colour
of callus was green and friable in nature. Other
hormonal combinations in this case did not induce
callus. These results suggested that the problems which
affect seed germination in the natural
environment are also reflected on germination in the
Murashige and Skoog medium and in callus
induction. Vulnerability to fungal attack is a
limitation for successful callus induction and germination in the culture room. More research under improved sterile
conditions is needed to improve callus
percentage for seedling multiplication.
Author
(s) Details
Dr. Washa B. Washa
Mkwawa University College of Education (MUCE), Iringa, Tanzania and A
Constituent College of the University of Dar es Salaam, Tanzania.
View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/268
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