Selection and Antagonistic Activity of Potentially Probiotic Lactic Acid Bacteria against Vibrio parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease (AHPND) in White-leg Shrimp | Chapter 5 | Cutting Edge Research in Biology Vol. 6

 The present study proposed to select the strains of Lactic Acid Bacteria (LAB) that can antagonize Vibrio parahaemolyticus  and to use these strains in forbiding AHPND in culture insignificant. The research contents espoused the following four things: (1) Isolating, classifying and hide LAB strains based on determinations of semantic, physiological and biography-chemical traits; (2) The investigations of their opposition against V. parahaemolyticus of LAB strains by using the agar well spread method; (3) The belongings of dietary LAB preservatives on the death rate and fighting of the white-lap shrimps to AHPND; (4) Identifications of the LAB strains that have excellent resistance to AHPND in silvery-leg insignificant. By using the agar well spread method, the judgments indicated that five at a distance 94 isolated LAB strains granted the highest antagonizing capability against V. parahaemolyticus strain with an hindrance zone width oscillating from 17.5 to 18.5 mm.  Also, the death rates of the silvery leg invertebrate in the treatments on that the shrimps were disputed with AHPND strain and augment with the LAB1, LAB2 and LAB5 enhanced feed, were significantly inferior that in the positive control situation (54.43%), but not significantly above that in the negative control treatment (12.23%) in the ending of 16 days after situation. However, the histological images of insignificant hepatopancreas indicated that the contaminated rate by V. parahaemolyticus significantly discounted from 60.0% to 11.1% in shrimps augment with LAB-augmented feed as compared to those in the definite control treatment (nonentity were challenged accompanying the harmful microorganisms without augmenting with LAB-supplement feed). To establish the identification of above five high-quality LAB strains, sequencing 16S rRNA gene was administered. The result showed that LAB1 strain was Lactobacillus plantarum, LAB2 strain was Pediocococcus pentosaceus 1, LAB3 strain was L. garvieae, LAB4 strain was P. pentosaceus 2 and LAB5 strain was L. fermentum.

Author(s) Details:

Linh Nguyen Thi Truc,
Tra Vinh University, no. 126, Nguyen Thien Thanh Street, Tra Vinh City, Tra Vinh Province 87000, Viet Nam.

Hai Luu Thi Thuy,
a Tra Vinh University, no. 126, Nguyen Thien Thanh Street, Tra Vinh City, Tra Vinh Province 87000, Viet Nam.

Tuu Nguyen Thanh,
Tra Vinh University, no. 126, Nguyen Thien Thanh Street, Tra Vinh City, Tra Vinh Province 87000, Viet Nam.

Nghia Nguyen Trong,
Aquaculture Pharmacy Company Limited, 149/41, Hoang Van Thu Street, An Cu Ward, Ninh Kieu District, Can Tho City 94000, Viet Nam.

Phu Truong Quoc,
Department of Aquatic Pathology, College of Aquaculture and Fisheries, Can Tho University, Ninh Kieu District, Can Tho City 94000, Viet Nam.

Please see the link here: https://stm.bookpi.org/CERB-V6/article/view/10220