Hydrophilic Interaction Liquid Chromatography (HILIC) is a type of common phase chromatography that acts with a very polar fixed phase and a mainly organic mobile stage. Hydrophilic interaction is conspicuous once the mobile point includes not completely 60% organic solvent. The system of HILIC involves partitioning betwixt a water-enriched layer namely partially disabled on the hydrophilic stationary phase and the less cold mobile aspect. Instrumentally speaking, a chromatographic system for HILIC chromatography is nearly equivalent to an RPLC (Reversed Phase Liquid Chromatography) system. The fixed phase in HILIC columns is hydrophilic and commonly also accused, at least in some pH ranges. Compounds divided on the column communicate with the stationary chapter and are generally more powerfully retained the more hydrophilic the compound. It is used to determine the concentrations of dimethindene maleate in restricted gel, zanamivir in informer and monkey plasma, and tromethamine as the counterion in an investigational pharmaceutical seasoning.
Author(s) Details:
Rupali Kirtawade,
Government College of Pharmacy, Karad,
Maharashtra, India.
Pallavi
Salve,
Government
College of Pharmacy, Karad, Maharashtra, India.
Anita Kulkarni,
Government College of Pharmacy, Karad, Maharashtra, India.
Pandurang Dhabale,
Government College of Pharmacy, Karad, Maharashtra, India.
Please see the link here: https://stm.bookpi.org/NAPR-V1/article/view/10305
No comments:
Post a Comment