Negative Regulation of Wnt/β-catenin Signaling is Blocked by Wnt Signaling Component Protein Dishevelled1 Binding Aptamer (WD-Aptamer): A Recent Study | Chapter 1 | Highlights on Medicine and Medical Science Vol. 17

 Dishevelled1 (Dvl1) is made up of three domains that have a function in Wnt/-catenin signalling. The DIX domain is responsible for Dvl1 polymerization, whereas the DEP domain is responsible for Dvl1 binding to other signal transduction molecules and the PDZ domain is responsible for binding to the membrane-bound receptor Frizzled. CXXC5 is one of several proteins that connect to Dvl1, and when these two proteins interact, they inhibit Wnt/-catenin signalling. Signaling via Wnt/-catenin is important for bone production, hair renewal, and wound healing.

We created WD-Aptamer, a DNA aptamer that precisely binds to the Dvl1 PDZ domain of the Wnt signalling component protein Dvl. The WD-Aptamer that binds to the Dvl1 PDZ domain was created using an in vitro selection process known as SELEX (Systematic Evolution of Ligands by EXponential Enrichment). With a Kd of 284.8 nM, the WD-Aptamer binds to the Dvl1 PDZ domain with excellent specificity and affinity. The WD-Aptamer blocks negative regulation of Wnt/-catenin signalling by inhibiting CXXC5 and Dvl1 binding.

Author (S) Details

Sooho Choi
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Ji Hyun Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Yunseok Heo
Structural Biochemistry & Molecular Biophysics Laboratory, Department of Biochemistry, College of Life Sciences & Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

Eun-Sun Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Solip Choi
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Weontae Lee
Structural Biochemistry & Molecular Biophysics Laboratory, Department of Biochemistry, College of Life Sciences & Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

Jeong Hoon Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

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