Marine Biopolymers in Advanced Wound Care: Mechanisms and Clinical Applications | Chapter 10 | Research Perspective on Biological Science Vol. 10

 

Wound healing is a complex physiological process that can be disrupted by pathological conditions, causing chronic wounds when they represent a significant burden on health systems. Marine-based biopolymers, particularly chitosan and alginate, have attracted attention as wound care biomaterials due to their properties, such as being biocompatible and biodegradable with intrinsic bioactivities. Nutritional interventions are also predominantly carried out to promote the wound-healing process, and this chapter provides a detailed panorama on the clinical uses of chitosan and alginate, which are already utilised in wound healing amidst nutritional interventions as observed. The present study describes mechanisms of action along with haemostatic, antimicrobial, anti-inflammatory and regenerative effects. The chapter summarises the experimental and clinical evidence, including randomised controlled trials, showing that chitosan-based dressings are effective in promoting healing of chronic ulcers such as diabetic foot ulcers. Instead, with clinical experience, alginate dressings used in high-exudate wounds are shown as beneficial; yet, systematic reviews revealed that this type of dressing did not promote healing rates better than other alternative modern dressings. It also addresses the synergetic effects of a composite dressing that consists of chitosan and alginate, as they demonstrate superior mechanical and therapeutic features in preclinical models. Oral chitosan and alginate are nutritional supplements that are generally believed to promote wound healing (although this is speculative without clear clinical evidence). Future studies could enable the development of “smart” dressings, and larger, more definitive clinical trials are required to realise the true potential of these marine-derived biomaterials in advanced wound care.

 

 

Author(s) Details

A. A. Zubair
PG and Research Department of Aquaculture and Fishery Microbiology, MES Ponnani College, Ponnani South, Malappuram, India.

 

Please see the book here :- https://doi.org/10.9734/bpi/rpbs/v10/7253

Dermatological Applications of Punica granatum: Phytochemistry and Therapeutic Potential in Skin Disorders |Chapter 7 | Pharmaceutical Science: New Insights and Developments Vol. 9

 

Pomegranate (Punica granatum) is a popular fruit known for its rich nutritional value and diverse bioactive compounds. The fruit has many potential health benefits, and it has been evaluated in clinical trials for different diseases. There are limited studies investigating the comprehensive use of pomegranate in cosmetic and dermatological applications. This chapter presents a comprehensive review of the dermatological applications and therapeutic potential of Punica granatum, with a focus on its phytochemistry and mechanisms of action. The increasing use of botanical extracts in skincare and cosmetic formulations underscores the relevance of Punica granatum, a plant native to Iran. A structured literature search was conducted across various databases, including PubMed, ScienceDirect, Springer, and Google Scholar, to evaluate evidence from preclinical and clinical studies. Key findings indicate that pomegranate extracts and their bioactive compounds—particularly punicalagin, ellagic acid, and anthocyanins—exhibit significant antioxidant, anti-inflammatory, antimicrobial, and wound-healing properties. Specific dermatological benefits explored include the promotion of wound repair, inhibition of acne pathogenesis, protection against UV-induced photoaging, skin brightening, and management of inflammatory skin conditions such as atopic dermatitis. This review consolidates current scientific research to highlight the promising role of Punica granatum as a multifunctional agent in the treatment and prevention of skin disorders, supporting its continued integration into dermatological and cosmetic products.

 

Author(s) Details

Farshad Akbarnejad
Dr. Akhavi Laboratory Co, Scientific Department, Tehran, Iran.

 

 

Please see the book here :- https://doi.org/10.9734/bpi/psnid/v9/6868

Prominent Use of Malva sylvestris L in Dermatology | Chapter 8 | Research Perspective on Biological Science Vol. 9

 

Malva sylvestris L. (common mallow) is a flowering plant belonging to the Malvaceae family. The plant has laxative, diuretic, spasmogenic and laxative properties. This plant has a rich history in traditional medicine across Asia, Europe, and Africa, and presents a compelling natural therapeutic agent for a wide range of dermatological conditions. Among the various species used in herbal medicine, M. sylvestris stands out for its diverse usage, which has been documented since 3000 BC. In Iran, M. sylvestris is recognised as an edible herb and has been consumed raw in various vegetable salads; however, it is most commonly used as a medicinal herb. This review consolidates scientific evidence to validate its traditional use in treating wounds, eczema, atopic dermatitis, and skin infections. The therapeutic efficacy of M. sylvestris is primarily attributed to its diverse profile of bioactive phytoconstituents. Key among these are mucilaginous polysaccharides, which provide emollient and soothing effects for sensitive skin and irritations; flavonoids (e.g., gossypetin, malvidin 3-glucoside) and phenolic compounds, which exert potent antioxidant and anti-inflammatory activities by scavenging free radicals and modulating inflammatory mediators like PGE₂; and tannins and essential oils, which contribute to its significant antibacterial effects against pathogens such as Staphylococcus aureus. Preclinical and clinical studies demonstrate that topical applications of M. sylvestris extracts promote wound healing by enhancing collagen deposition and fibroblast proliferation, reduce inflammation and pruritus in atopic dermatitis, and accelerate burn wound contraction, often showing superior efficacy compared to standard treatments like silver sulfadiazine. The convergence of its demulcent, antibacterial, anti-inflammatory, and antioxidant properties, underpinned by its distinct phytochemical composition, justifies its traditional use and positions M. sylvestris as a versatile and effective candidate for integrative dermatological therapy. Future studies, including well-designed clinical trials and standardised extract formulations, are required to validate the therapeutic efficacy of Malva sylvestris in dermatological conditions.

 

 

Author(s) Details

Farshad Akbarnejad
Scientific Department, Rayka Shimi Saba Pharmaceutical & Hygienic Knowledge-Based Co., Tehran, Iran.

 

Please see the link:- https://doi.org/10.9734/bpi/rpbs/v9/6800

 

A Novel Polyherbal Formulation- Sarkaraikolli Hastens Wound Healing Activity with Antidiabetic Potential | Chapter 3 | Pharmaceutical Science: New Insights and Developments Vol. 8

 

Diabetes is a chronic group of metabolic diseases characterised by the constantly elevated blood glucose levels, which leads to the glycation of body proteins, which may further cause serious complications. In recent years, the use of complementary medicine has seen significant growth, particularly in dietary interventions and traditional plant-based therapies derived from systems such as Ayurveda. No systematic studies have been reported for its wound healing and anti-diabetic properties of Sarkaraikolli. An effort has been made to establish the wound healing and anti-diabetic properties of the polyherbal formulation Sarkaraikolli. In this model, animals were administered with test and standard samples by the oral route. Frequently blood samples were taken and examined for blood glucose levels, which were assessed using a glucometer. The animals were sacrificed 2 hours on the 21st day after the treatment by cervical dislocation, and biochemical studies were performed. The blood samples were collected, and serum was separated by centrifugation at 3000 rpm for 15 minutes after a retro-orbital puncture. Different procedures were performed for the determination of liver enzyme tests. In the model of anti-diabetic animals were evenly divided into 5 groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4, and 5 were the treatment groups which were simultaneously treated with standard, 250 and 500 mg/kg extract respectively after glucose loading. Sarkaraikolli possesses significant anti-diabetic and wound healing activity when compared to the conventional medicine like Glibenclamide. Significant anti-diabetic action is visible due to lower blood glucose levels and maintenance of steady body weights. Sarkaraikolli wound healing activity is demonstrated by higher wound healing activity as compared to the standard medication. The biochemical parameters like SGOT, SGPT and ALP were determined by using the analytical kits. The results shown by the treatment of Sarkaraikolli were significant and are closer to the control group. This research work proved that Sarkaraikolli possesses significant anti-diabetic and wound-healing activity. When compared to conventional medicine, Glibenclamide, a significant anti-diabetic action is visible due to lower blood glucose levels and maintenance of steady body weights. Sarkaraikolli's wound healing activity is demonstrated by higher wound healing activity when compared to the standard drug (Nitrofurazone).

 

 

Author(s) Details

S. Sundar
Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Andhra Pradesh, India.

 

A. Bhavana
Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Andhra Pradesh, India.

 

N. K. S. Neeraja
Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Andhra Pradesh, India.

 

D. Prasanna
Department of Pharmaceutical Chemistry, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Andhra Pradesh, India.

 

R. Sunitha
Department of Pharmacognosy, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Andhra Pradesh, India.

 

 

Please see the book here:- https://doi.org/10.9734/bpi/psnid/v8/6122

Plant-Based Natural Compounds for Wound Healing: A Comprehensive Review | Chapter 6 | Research Perspectives of Microbiology and Biotechnology Vol. 4

The study presents a comprehensive assessment of plant-sourced natural compounds for wound healing, encompassing their chemical origins and the biological processes involved in their therapeutic effects on wound healing. Wound healing involves multiple stages, and any interruption during this sequence can result in improper wound healing. Restoration of the structure and function of damaged tissues, which is an intricate process, is governed by the discharge of multiple cytokines and growth factors at the site of injury. Wound healing may benefit greatly from a variety of plants or compounds derived from plants with high concentrations of antioxidants as well as anti-inflammatory, immunomodulatory, and antibacterial properties.

 

Author(s) Details

Monika

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, India.

Surabhi Bajpai

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, India.

Rakesh Mishra

Institute for Stem Cell Biology and Regenerative Medicine (inStem), NCBS Campus, Bengaluru -560065, Karnataka, India.

 

Please see the link:- https://doi.org/10.9734/bpi/rpmab/v4/774

Plant-Based Natural Compounds for Wound Healing: A Comprehensive Review | Chapter 6 | Research Perspectives of Microbiology and Biotechnology Vol. 4

 

The study presents a comprehensive assessment of plant-sourced natural compounds for wound healing, encompassing their chemical origins and the biological processes involved in their therapeutic effects on wound healing. Wound healing involves multiple stages, and any interruption during this sequence can result in improper wound healing. Restoration of the structure and function of damaged tissues, which is an intricate process, is governed by the discharge of multiple cytokines and growth factors at the site of injury. Wound healing may benefit greatly from a variety of plants or compounds derived from plants with high concentrations of antioxidants as well as anti-inflammatory, immunomodulatory, and antibacterial properties.

 

 

Author(s) Details

 

Monika

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, India.

Surabhi Bajpai

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, India.

Rakesh Mishra

Institute for Stem Cell Biology and Regenerative Medicine (inStem), NCBS Campus, Bengaluru -560065, Karnataka, India.

 

Please see the link:- https://doi.org/10.9734/bpi/rpmab/v4/774

The Extracellular Matrix (ECM) and Wound Healing: A Review | Chapter 9 | Research Advances in Microbiology and Biotechnology Vol. 8

 The purpose concerning this article search out summarize current and archival literature demonstrating the act of the Extracellular Matrix (ECM) in the various stages of the wound curative cascade. ECM is a three-dimensional foundation that is about all human tissues. It is made up of a variety of proteins, containing collagens, elastin, and minor amounts of structural proteins. The ECM has existed shown in studies to aid in basic adhesion, fabric anchoring, cellular indicating, and cell conscription. The ECM is affected now and then of integumentary injury or damage, either acute or chronic. The ECM is founded and ideally restored to its native condition by a order of overlapping projects known as the wound healing stages: hemostasis, inflammation, increase, and remodeling. The authors trust that more study should be finished to not only use the structural components of the ECM but too to imitate the corporal structure.

Author(s) Details:

Robert B. Diller,
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA.

Aaron J. Tabor,
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA.

Please see the link here: https://stm.bookpi.org/RAMB-V8/article/view/12629

Exceptional Allosteric Regulation of Methylation Enzymes | Chapter 5 | Novel Research Aspects in Medicine and Medical Science Vol. 4

 The objective concerning this study is to raise youngster exceptional importance of methylation enzymes (MEs) in the managing of cell copy of differentiation to subject these enzymes to double allosteric rules and to pay attention to destabilization of abnormal MEs as a fault-finding allosteric regulation of malignancy therapy. MEs are a having three of something enzyme complex consisting of methionine adenosyltransferase (MAT)-methyltransferase (MT)-S-adenosylhomocysteine hydrolase (SAHH), that play an essential role to organize cell copy and differentiation in response to allosteric rule. MEs are subject to double allosteric rules, one at the individual enzymes and another at the something which incites activity complex.  On the individual enzymes, SAHH is the receptor of steroid hormones or related allosteric regulators that dictates the optimum of development and differentiation. Allosteric managers play an essential role to maintain organic optimum to prevent hazardous extreme frequently to result in the display of clinical manifestations. On the enzyme complex, the partnership with telomerase changes kinetic possessions of MAT and SAHH to alter the managing in favor of progress. Primitive stem cells such as rudimentary stem cells (ESCs) and parent stem cells (PSCs) express telomerase.  Cells accompanying abnormal MEs have a great benefit on growth. The character creates chemo-following as an allosteric regulation to destabilize unusual MEs to keep containers with uncommon MEs under control.Wound healing demands the proliferation and the terminal distinction of PSCs. The functionality of chemo-following dictates the success of wound healing. If the use of chemo-surveillance has happened damaged on account of pathological conditions, before the terminal differentiation of PSCs will experience to result in very weighty illnesses such as fabric fibrosis, dementia and tool failure.  Wound unhealing can further force PSCs to evolve into cancer stem containers (CSCs) through silencing of TET-1 catalyst to escape contact inhibition that limits the increase of PSCs. The proliferation of CSCs cannot heal the wound by way of the collapse of chemo-surveillance, that are then strained to progress to fast growing cancer containers (CCs) by the incitement of oncogenes and/or the inactivation of suppressor genes. Obviously, the best approach of cures of illnesses due to wound unhealing search out restore the service of chemo-surveillance.  CDA formulations are, so, the best drugs for cancer analysis to fulfill tumor moonshot and to win the war on cancer.

Author(s) Details:

Ming C. Liau,
CDA Therapeutics, Inc., 3308 Sky Run Court, Missouri City, TX 77459, USA.

Christine L. Craig,
CDA Therapeutics, Inc., 3308 Sky Run Court, Missouri City, TX 77459, USA.

Linda L. Baker,
CDA Therapeutics, Inc., 3308 Sky Run Court, Missouri City, TX 77459, USA.

Please see the link here: https://stm.bookpi.org/NRAMMS-V4/article/view/12085

A Drastic Change of Cancer Leaderships to Save Desperate Cancer Patients | Chapter 6 | New Advances in Medicine and Medical Science Vol. 7

 The objective concerning this study is to find solutions to sustain terminal cancer cases desperate to stay alive. Tragically, individual of the outcomes of World War II was the adaptation of cytotoxic tumor therapy. Bombs holding poisonous sulphur mustard gas were secondhand during the war. All of the victims of poisonous gas bombs had curbed lymphocyte counts in their blood samples, which stimulated oncologists to utilise hazardous substances to treat leukaemia sufferers. Early stage cancer grant permission benefit from cytotoxic cancer therapy when the use of chemo-surveillance is not yet giving small ground for hope damaged. Our studies strongly signify that cancer is carefully related to wound healing. Cancer stands as a consequence of wound unhealing. Untreatable cancers are loaded accompanying CSCs such as basic brain tumors and terminal cancer subjects above described. CDA formulations are the best cures to solve aforementioned desperate cases.There is no mechanism inside the health profession to correct the mistakes created by cancer establishments. President Biden was really committed to save tumor patients to declare tumor moonshot initiative. He has the expert to replace leaderships of federal fitness agencies to correct the mistakes of cancer establishments to preserve desperate malignancy patients.

Author(s) Details:

Ming C. Liau,
CDA Therapeutics, Inc. 3308 Sky Run Court, Missouri City, TX 77459, USA.

Christine L. Craig,
CDA Therapeutics, Inc. 3308 Sky Run Court, Missouri City, TX 77459, USA.

Linda L. Baker,
CDA Therapeutics, Inc. 3308 Sky Run Court, Missouri City, TX 77459, USA.

Please see the link here: https://stm.bookpi.org/NAMMS-V7/article/view/11036

Perinatal Tissues to Study In vitro Mechanisms of Inflammation and Angiogenesis: Novel Insight on Diabetic Non-Healing Wound | Chapter 4 | Research Highlights in Disease and Health Research Vol. 3

 Impaired wound restorative is individual of the main diabetes mellitus complications befriended to various disadvantageous visage such as system which controls organization of a supporting-instigative and pro-oxidative atmosphere in addition to weakened peripheral ancestry flow and angiogenesis. Unfortunately, persuasive medicines are now lacking. Recently, with perinatal products, Amniotic Membrane (AM) has explained encouraging results in wound administration.Therefore, in this place the potential effect of AM on endothelial containers unique from the umbilical rope tone of gestational diabetes impressed women (GD-HUVEC), have happened examined. Indeed, GD-HUVEC have proved a pro-instigative phenotype and lower ship establishment on Matrigel distinguished to control HUVEC (C-HUVEC), thus exhibiting a valuable model for learning the antagonistic-inflammatory act of AM and neovascularization of incessant non-restorative wounds. The antagonistic-inflammatory features of AM have existed determined in Tumor Necrosis Factor- α (TNF- α) pre-stimulated containers utilizing a monocyte-endothelium cling assay and evaluating vascular cling fragments verbalization and sheet exposure, in addition to Nuclear Factor kappa-light-chain-enhancer of stimulated B containers (NF-kB) expression and basic fluctuation and Nitric Oxide (NO) bioavailability. Moreover, hose establishment ability was intentional in AM-acted C- and GD-HUVEC.The verdicts indicated that AM considerably diminished TNF- α aroused monocyte-endothelium interaction and sheath uncovering of the Vascular container and Intracellular stickiness molecules (VCAM-1 and ICAM-1, individually) in two together C- and GD-HUVEC. Strikingly, AM situation significantly upgraded hose-like construction interconnections in GD-HUVEC.Overall, these results display that, in our in vitro container model, AM attenuates TNF- α -raised swelling and improves angiogenesis, perhaps through the timbre of NO bioavailability, that plays a key role in the vascular equilibrium balance. This study desires that AM incessant wound curative improvement grant permission develop endothelial correct management, so interpreting allure dispassionate benefit on diabetic foot ulcers.

Author(s) Details:

Caterina Pipino,
Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio Chieti-Pescara, StemTeCh group, Chieti, Italy.

Ilaria Cappellacci,
Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio Chieti-Pescara, StemTeCh group, Chieti, Italy.

Javier Stelling-Ferez,
Regeneration, Molecular Oncology and TGFß, IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120, El Palmar Murcia, Spain and Department of Nutrition and Food Technology, UCAM, 30107, Guadalupe, Murcia, Spain.

Angel Bernabe-Garcia,
Regeneration, Molecular Oncology and TGFß, IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120, El Palmar Murcia, Spain.

Domitilla Mandatori,
Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio Chieti-Pescara, StemTeCh group, Chieti, Italy.

Nadia Di Pietrantonio,
Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio Chieti-Pescara, StemTeCh group, Chieti, Italy.            

Carlos Navalon,
Regeneration, Molecular Oncology and TGFß, IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120, El Palmar Murcia, Spain.

Francisco Jose Nicolas,
Regeneration, Molecular Oncology and TGFß, IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120, El Palmar Murcia, Spain.

Assunta Pandolfi,
Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio Chieti-Pescara, StemTeCh group, Chieti, Italy.

Please see the link here: https://stm.bookpi.org/RHDHR-V3/article/view/9782

Therapeutic Drug Monitoring of Gentamicin in a Patient with Delayed Wound Healing | Chapter 6 | Perspective of Recent Advances in Medical Research Vol. 12

 Gentamicin, secondhand for the treatment of weighty gram-negative contaminations, has a narrow therapeutic index. This property can lead to various side effects, accordingly, therapeutic drug listening (TDM) is important in gentamicin therapy.This division aims to report the pharmacokinetics of gentamicin when using a previously-daily drug regimen.The patient, a 78-period-old male, weighed 78 kg and was 1.75 m tall. He was introduced into the hospital accompanying a wound infection provoked by chronic appendage ischaemia. A once-daily drug of gentamicin (360 mg by infusion over 30 record) in combination accompanying flucloxacillin was prescribed to this patient. A gentamicin ancestry sample was taken every day for four days: Day 1 = 1.4 mg/L, Day 2 = 2.1 mg/L, Day 3 = 2.1 mg/L, Day 4 = 1.6 mg/L. The patient’s antitoxin creatinine was 88 µmol/L during the four days. To decide the dosage and break for gentamicin administration, the Hartford nomogram was working. A once-day-to-day dosing regimen of gentamicin can bring about the clinical and bacteriological cure of contamination. In addition, the Hartford nomogram is a reliable finish for keeping path of gentamicin's once-daily application schedule.

Author(s) Details:

Saeed Fahad Algahtani,
Department of Clinical Pharmacy, Infectious Disease Pharmacist, Prince Sultan Armed Forces Hospital In Madinah, Saudi Arabia.

Fahad M. Alqahtani,
Drug Information and Poisoning Center, Al Kharj Military Industries Corporation Hospital, Saudi Arabia.

Mohammed Qahl,
Department of Pharmacy, Northern Area Armed Forces Hospital, Saudi Arabia.

Please see the link here: https://stm.bookpi.org/PRAMR-V12/article/view/9660

King Alferd’s Mushroom (Daldinia concentrica) - An Investigation on its Cutaneous Wound Healing Activity from the Indigenous Knowledge of Tribes of Sirumalai Hills, Tamilnadu, India | Chapter 5 | Current Overview on Pharmaceutical Science Vol. 6

 The current study is an attempt to conduct an ethnomycological grow quickly survey among the paliyar tribals of five conclusions in the Sirumalai hills. Daldinia concentrica, a grow quickly used by tribals for wound restorative, was tested in vivo in a informer model. Wild mushroom likely to grow on areas accompanying high dampness and rainfall.  The survey revealed that 92% of the accused consume sprout as food and four mushrooms are secondhand in ethnomedicine in the study area that is to say, Daldinia concentrica, Calvatia gigantean, Termitomyces microcarpus and Podaxis pistillaris. Daldinia concentrica for wound healing, is the first ethnomycological report, so the further study on Daldinia  found that the mushroom sample resides of alkaloid 0.97±0.07mg/kg, flavanoid 2.97±0.12mg/kg, phenol 0.32±0.01mg/kg, tannin 0.17±0.02mg/kg, terpenoid 0.07±0.01mg/kg and saponin 0.09±0.02mg/kg; and it’s in vivo wound healing activity was raise to be superior around 75 – 87% distinguished to that of the commercial balm neosporin. The activity maybe attributed to the cooperative effect of phytochemicals found in bureaucracy. Our findings clear up the traditional use of mushrooms in a assortment of treatments.

Author(s) Details:

Sumathy Raj,
School of Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore, Tamil Nadu, India.

Rajeshwaran Thangaraj,
Department of Biology, The Gandhigram Rural Institute – Deemed University, Gandhigram, Dindigul, Tamilnadu, India.

Kumuthakalavalli Renganathan,
Department of Biology, The Gandhigram Rural Institute – Deemed University, Gandhigram, Dindigul, Tamilnadu, India.

Please see the link here: https://stm.bookpi.org/COPS-V6/article/view/9467

Mesenchymal Stem Cells and Secretome Accelerated Wound Healing in a Rodent Model| Chapter 10 | Perspective of Recent Advances in Medical Research Vol. 7

 Mesenchymal stem containers (MSCs) have happened proved to play a biological duty in wound curative. There have existed few studies on the curative effect of the secretome, that is containing many biological determinants emitted by MSCs. The aim concerning this study search out compare the healing belongings of secretome against MSCs on wound restorative. Green blooming protein branded adipose-derivative MSCs (GFP-ADMSCs) or secretome was introduced in the complete-denseness skin extraction model on SD rats. The wound restorative process was determined by scheming the restorative rate and operating histological examinations on skin surgical procedure. The cell being, increase, and flexibility of informer dermal fibroblasts were distinguished after miscellaneous situations. The level of nitric group of chemical elements (NO) and instigative cytokines in macrophages was contingent upon NO assay and ELISA. On day 5 and epoch 14, both MSCs and secretome increased the wound curative, secretome further embellished the process. GFP-MSCs were found 10 days subsequently the transplant. MSCs and secretome situations weakened the levels of IL-6 and TNF -� hereditary. After situation, the verbalizations of VEGF and PCNA increased, accompanying a greater force of VEGF noticed in secretome-introduced fabric.After the treatment, FR's container animation and conception were notably pushed. The scratch test told that secretome hastened up the rate of wound curative. Although macrophage absorption was considerably reduced by Secretome, the amount of NO released by macrophages was unsophisticated. The research granted that MSCs and MSC-derivative secretome upgraded the beginning stages of wound healing.  Secretome embellished the restorative belongings by advancing fibroblast increase and transfer while suppressing the angering reaction.

Author(s) Details:

Hui Ma,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Ping Kuen Lam,
HK Stem Cell Biotechnology Group Ltd, Hong Kong, China and Department of Surgery, Chow Tai Fook-Cheng Yu Tung Surgical Stem Cell Research Center, The Chinese University of Hong Kong, Hong Kong, China.

Wing Sum Siu,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Cindy See Wai Tong,
Department of Surgery, Chow Tai Fook-Cheng Yu Tung Surgical Stem Cell Research Center, The Chinese University of Hong Kong, Hong Kong, China.

Kin Ki Yan Lo,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Chi Man Koon,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Xiao Xiao Wu,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Xiang Li,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Wen Cheng,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Wai Ting Shum,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Ping Chung Leung,
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Please see the link here: https://stm.bookpi.org/PRAMR-V7/article/view/9322

Wound Healing Metabolites as the Right Medicines for the Therapy of Diseases Arising due to the Collapse of Chemo-Surveillance | Chapter 6 | Current Overview on Disease and Health Research Vol. 1

This study's goals were to investigate the mechanics of wound healing and look for wound healing metabolites as potential treatments for illnesses brought on by the failure of chemo-surveillance, the majority of which were incurable. Cancer evolution and wound healing have many similarities, and progenitor stem cells (PSCs) play a significant role in both processes. Wound healing requires PSC proliferation and terminal differentiation (TD). Wounds cause biological and immunological reactions. In order to create ProstaGlandins (PGs), which are active Differentiation Inducers, Arachidonic Acid (AA) is released from membrane-bound phosphatidylinositol as part of the biological response (DIs). The DIs AA and PGs promote wound healing. Tumor Necrosis Factor (TNF), also known as cachectin due to its ability to create the symptom of cachexia, is produced as a result of an immunological reaction. Cachexia is a poor indication of wound healing because TNF can lead to an increase in the excretion of low molecular weight metabolites in the urine, resulting in the loss of metabolites necessary for wound healing. As a result, chemo-functionality surveillance is essential for assessing how well wounds heal. A wound can always heal without leaving a scar if the chemo-surveillance system functions as well in cancer patients as it does in healthy individuals. However, if the effectiveness of chemo-surveillance has been compromised by pathological illnesses that are generating cachexia symptoms, wound healing may be hampered, leaving an unsightly scar. alternatively, in the case of irreversible brain injury, dementia; in the event of Covid-19 infection, tissue fibrosis; or, in the case of malignancy, the most terrifying scenario. In relation to aesthetic surgery and regenerative medicine, ugly scars on the visible surface are a medical problem. Studying wound healing can assist repair wounds without leaving scars and provide a more effective treatment plan for illnesses brought on by the breakdown of chemo-functionality.

Author (s) Details:

Ming C. Liau,
CDA Therapeutics, Inc. 3308 Sky Run Court, Missouri City, TX 77459, USA.

Christine Liau Craig,
CDA Therapeutics, Inc. 3308 Sky Run Court, Missouri City, TX 77459, USA.

Please see the link here: https://stm.bookpi.org/CODHR-V1/article/view/7426

Treatment of Skin Wounds in Old Animals Using Mesenzymal Progenitor Cells and Polymer Gel Compositions| Chapter 14 | Issues and Developments in Medicine and Medical Research Vol.11

The goal of this study was to see how successful progenitor mesenchymal stem cells were as injections and as part of a polymer hydrogel for wound healing in elderly animals.

Materials and Methods: On the backs of 9-month-old white Wistar rats, fixed-size wounds (average area of 135.8 mm2) were simulated. On the day of the modelling, MSC were extracted from a human umbilical cord and injected into the wounds once (SC group). MSC were administered externally on a regular basis as one of the components of the polymer hydrogel in other animals (Polymer sc group). Examining undamaged contralateral wounds on the opposite side of the same animal's back was used to assess the cells' systemic effect (control sc and Control Psc groups, respectively). The Control 0 group included the intact reference wounds. Dynamic analysis of the wound region was carried out. The collagen fibres' maturity, the epidermal layers, and the number of fibroblasts and leukocytes in different regions of the wounds were all assessed using descriptive microscopy.

The administration of MSCs, both locally and systemically, promoted wound repair. During the acute inflammatory phase, the method and site of application had little effect on the dynamics of wound healing (up to 3 days). Polymer sc was shown to be the most effective in the end. A reduction in leukocyte infiltration in wound centres (Polymer sc and SC groups) and edges (all groups, with the greatest extent in the Polymer sc group) indicated MSC's anti-inflammatory impact. The proliferative phase, which shows itself as increased fibroblast number and collagen production, was observed in the Control Psc group, particularly in the Polymer sc group.

Conclusion: MSC therapy improves and speeds wound healing in a variety of ways, even in elderly animals. In the Polymer sc category, the best results were achieved.

Author(s) Details:

Natalia Manturova,
Department of Plastic and Reconstructive Surgery, Cosmetology and Cell Technologies, Pirogov Russian National Research Medical University, Moscow, Russia.


Victor Stupin,
Department of Hospital Surgery no 1, Pirogov Russian National Research Medical University, Moscow, Russia.


Ekaterina Silina,
Institute of Biodesign and Modeling of Complex Systems, I. M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.

Please see the link here: https://stm.bookpi.org/IDMMR-V11/article/view/6086

Study on Dietary Considerations of Wound Healing in Ayurveda | Chapter 02 | Emerging Challenges in Agriculture and Food Science Vol. 3

 Because of a significant increase in the number of traumatic cases, wound healing has become a burning problem in a surgical practise. Nutrition has an important role in wound healing and wound care, and nutritional support should be regarded a key component of wound care. A wound causes a number of changes in the body, including changes in energy, protein, carbohydrate, fat, vitamin, and mineral metabolism, all of which can affect the healing process. Various Ayurvedic literatures, particularly the Sushrut samhita, which is claimed to be an old textbook of surgery in Ayurveda, have emphasised the diet for the person suffering from a wound, and the author stated that nutrition plays a critical role in wound healing. Sushrut–The father of surgery scientifically categorised it in a systemic manner, resulting in a plethora of clinical material and managerial methods that are still applicable today. Shalya tantra (Ayurveda science's surgical branch) is an important branch of ayurveda in which surgical and para-surgical techniques for the treatment of various ailments are discussed. The most important and well-known chapter of Shalya tantra is Vrana. Vrana (wound) is one of them, and it has been dealt with by humans since the dawn of civilization. Under the conditions, the first thing the men saw was the injury he had received from several sources, which had caused his vrana. Vrana is a terrible and frightening condition that affects people of all ages. The importance of a well-balanced diet in wound healing cannot be overstated. The purpose of this article is to learn about the significance of nutrition and dietetic issues during wound healing.

Author(S) Details

Sanjeev S. Tonni
Department of Swasthavritta, KLE Academy of Higher Education and Research (KAHER), Shri. B. M. Kankanawadi Ayurveda Mahavidyalaya and Hospital, Shahapur, Belagavi-590003, Karnataka, India.

Amruta A. Wali
Department of Swasthavritta, KLE Academy of Higher Education and Research (KAHER), Shri. B. M. Kankanawadi Ayurveda Mahavidyalaya and Hospital, Shahapur, Belagavi-590003, Karnataka, India.

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Study about Wound Healing, Evolution of Cancer and War on Cancer | Chapter 14 | Recent Developments in Medicine and Medical Research Vol. 8

 The impact of wounds on the progression and treatment of cancer is highlighted in this review. The goal of this review is to look into the process of wound healing and how it relates to cancer evolution and treatment. Proliferation and terminal differentiation (TD) of progenitor stem cells (PSCs), the progenitors of cancer stem cells, are required for wound healing (CSCs). It is not difficult to heal a wound. If the chemo-surveillance system is working properly, such as in healthy people who can keep a constant level of wound healing metabolites acting as differentiation inducers (DIs) and differentiation helper inducers (DHIs). Wounds are always properly healed without any effort on the part of the patient; all that is required is for the patient to relax and allow nature to cure the wound. Sutures and antibiotics are used as a last resort to speed up the healing process or avoid infection. The functionality of chemo-surveillance is momentarily harmed by an acute wound, which heals fast and returns to normal. Chronic wounds, such as persistent viral illnesses or long-term exposure to toxic substances, especially carcinogens, have a negative impact on chemo-surveillance functionality. Wound healing is hampered by the generation of inflammatory cytokines, which promote increased urine excretion of wound repair metabolites. It is relatively easy for PSCs to transition into CSCs if there aren't enough wound healing metabolites to stop PCSs from proliferating. To complete the change, all it takes is a single hit to silence the TET-1 enzyme, which is well within the reach of PSCs with abnormally active methylation enzymes (MEs). The activation of oncogenes or the silencing of oncogenes in CSCs can then lead to the development of faster growing cancer cells (AML). MDS and AML aren't the only ones who get cancer because their wounds don't heal properly. It's a rather typical occurrence. The war on cancer can be easily won if the combat is performed in accordance with nature's path for healing the wound, similar to the success of wound healing in healthy people without exerting any effort. As a result, using DIs and DHIs to restore chemo-surveillance functionality and anti-cachexia compounds like phenylacetylglutamine to prevent the loss of wound healing metabolites is the greatest way to win the war on cancer. The proliferation of cells with aberrant MEs, such as CSCs, PSCs, and all cancer cells, will thereafter be stopped by nature. Destruction as a method of killing cancer cells is clearly counterproductive. It causes more damage to the chemo-surveillance system's ability to inhibit the proliferation of cells with aberrant MEs. The failure of the destruction strategy to eliminate CSCs is a deciding factor in denying the destruction approach's ability to win the cancer fight.

Author(S) Details

Ming C. Liau
CDA Therapeutics, Inc, 3308 Sky Run Court, Missouri City, TX 77459, USA.

Linda Liau Baker
CDA Therapeutics, Inc, 3308 Sky Run Court, Missouri City, TX 77459, USA.

View Book:- https://stm.bookpi.org/RDMMR-V8/article/view/4573

Management of Large Periapical Lesion of Endodontic Origin with the Combination of Second Generation Platelet Extract and Xenograft | Chapter 3 | New Frontiers in Medicine and Medical Research Vol. 8

 The local response of bone at the apex of the tooth that develops following pulp necrosis or significant periodontal disease is known as periapical inflammatory lesion. Periapical surgery seeks to remove periapical disease in order to promote wound healing and bone and periodontal tissue regeneration. This case report aims to shed information on the utilisation of platelet-rich fibrin and bone transplant in the treatment of big periapical lesions. Platelet rich fibrin (PRF) is a fantastic tissue engineering product that has garnered a lot of traction due to its promising wound healing and bone induction results. In both cases in this case report, the bone graft employed was xenograft, which is identical to human cancellous bone and has both osteoinductive and osteoconductive qualities.

Objectives:

1. To remove tissues that are infected, inflammatory, necrotic, or granulation.

2. Decontaminate and disinfect the peri-apical field.

3. To use a regenerative surgical method with second generation Platelet extract and Xenograft to promote superior and rapidly progressive healing in the peri-apical region.

Author (S) Details

Pradnya Sunilkumar Nagmode
Department of Conservative Dentistry & Endodontics, India.

Balaji Sopanrao Kapse
Department of Conservative Dentistry & Endodontics, India.

Harshal Vinod Basatwar
Department of Conservative Dentistry & Endodontics, India.

Shubham Prakash Godge
Department of Conservative Dentistry & Endodontics, India.

Prachi Nishant Janbandhu
Department of Conservative Dentistry & Endodontics, India.

View Book :- https://stm.bookpi.org/NFMMR-V8/article/view/3106

Negative Regulation of Wnt/β-catenin Signaling is Blocked by Wnt Signaling Component Protein Dishevelled1 Binding Aptamer (WD-Aptamer): A Recent Study | Chapter 1 | Highlights on Medicine and Medical Science Vol. 17

 Dishevelled1 (Dvl1) is made up of three domains that have a function in Wnt/-catenin signalling. The DIX domain is responsible for Dvl1 polymerization, whereas the DEP domain is responsible for Dvl1 binding to other signal transduction molecules and the PDZ domain is responsible for binding to the membrane-bound receptor Frizzled. CXXC5 is one of several proteins that connect to Dvl1, and when these two proteins interact, they inhibit Wnt/-catenin signalling. Signaling via Wnt/-catenin is important for bone production, hair renewal, and wound healing.

We created WD-Aptamer, a DNA aptamer that precisely binds to the Dvl1 PDZ domain of the Wnt signalling component protein Dvl. The WD-Aptamer that binds to the Dvl1 PDZ domain was created using an in vitro selection process known as SELEX (Systematic Evolution of Ligands by EXponential Enrichment). With a Kd of 284.8 nM, the WD-Aptamer binds to the Dvl1 PDZ domain with excellent specificity and affinity. The WD-Aptamer blocks negative regulation of Wnt/-catenin signalling by inhibiting CXXC5 and Dvl1 binding.

Author (S) Details

Sooho Choi
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Ji Hyun Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Yunseok Heo
Structural Biochemistry & Molecular Biophysics Laboratory, Department of Biochemistry, College of Life Sciences & Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

Eun-Sun Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Solip Choi
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Weontae Lee
Structural Biochemistry & Molecular Biophysics Laboratory, Department of Biochemistry, College of Life Sciences & Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

Jeong Hoon Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

View Book :- https://stm.bookpi.org/HMMS-V17/article/view/2604