Disseminated tumour cells (DTCs) in the bone marrow (BM) have been found to be distant metastasis progenitors. The finding of DTCs in non-small cell lung cancer (NSCLC) will provide critical information on metastatic characteristics, as well as the possibility of uncovering new targets for NSCLC treatment. The study's purpose is to examine if DTC can be found in BM and to figure out how often BM involvement is in NSCLC patients, as well as how it affects the lymphocyte population in the BM. 62 bone marrow samples from NSCLC patients were examined using morphological and immunological methods. Flow cytometry (FACS Canto II, USA, Kaluza Analysis v2.1 software) was used to analyse DTCs. Monoclonal antibodies to CD45, EPCAM, CD133, lymphocyte populations CD3, CD4, CD8, CD19, CD20, CD16, and CD27 were directly labelled with various fluorochromes. In 43.5 percent of patients, EPCAM+CD45- (DTCs) were identified in the BM (threshold level: 1 cell per 10 million myelocaricytes). In 33.3% (9/27) of the instances, CD133+EPCAM+CD45-cells were discovered. DTC presence had no correlation with tumour size, lymph node status, or tumour stage. Stages IA and IIA had the highest rates of DTC detection: 60.7 percent and 58.3 percent, respectively. BM involvement was observed in 45 percent of instances of adenocarcinoma and 37 percent of samples of squamous cell carcinoma (p = 0.501). Highly differentiated tumours had a higher prevalence of DTCs (p = 0.023). There are no significant links between the presence of DTCs in the BM and the parameters of the myelogram. In 4 percent of BM involvement, the number of granulocytic lineage cells decreased (p = 0.036). With BM injury, the level of CD16 + CD4-NK-cells (p = 0.002) and CD27 + CD3 + T-cells (p = 0.015) subpopulations increased significantly. DTCs can be found in the BM of NSCLC patients, according to the findings. The BM accounted for 43.5 percent of the participants. DTCs are observed even in the early stages of NSCLC. A relationship between BM involvement and the degree of tumour differentiation was established. Squamous cell lung cancer exhibited a higher rate of BM involvement than adenocarcinoma of the lung. The link between DTCs and BM lymphocyte populations was discovered: CD16 + CD4-, CD27 + CD3+ subpopulations.
Author (S) Details
Stilidi Ivan
Federal State Budgetary Institute “N.N. Blokhin National medical research center of oncology” of the Russian Ministry of Health, Moscow; Kashyrskoe sh.24, Moscow, 115478, Russia and Pirogov N.I. Russian National Research Medical University of the Russian Ministry of Health, 1, Ostrovitianova st., Moscow, 117997, Russia.
Kononetz Pavel
Federal State Budgetary Institute “N.N. Blokhin National medical research center of oncology” of the Russian Ministry of Health, Moscow; Kashyrskoe sh.24, Moscow, 115478, Russia.
Chulkova Svetlana
Federal State Budgetary Institute “N.N. Blokhin National medical research center of oncology” of the Russian Ministry of Health, Moscow; Kashyrskoe sh.24, Moscow, 115478, Russia and Pirogov N.I. Russian National Research Medical University of the Russian Ministry of Health, 1, Ostrovitianova st., Moscow, 117997, Russia.
Tupitsyn Nikolay
Federal State Budgetary Institute “N.N. Blokhin National medical research center of oncology” of the Russian Ministry of Health, Moscow; Kashyrskoe sh.24, Moscow, 115478, Russia.
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