Development of Multivariate Calibration Technique for the Spectrophotometric Quantification of Ivermectin in Pharmaceutical Formulation | Chapter 2 | Challenges and Advances in Pharmaceutical Research Vol.1

 The goal of this abstract is to quantify ivermectin in pharmaceutical dosage form using a multivariate calibration technique.

Methods: To correlate the relationship between concentration and absorbance at seven different wavelengths, the multivariate calibration technique uses linear regression equations. Ivermectin's _max was discovered to be 245 nm. The data was analysed statistically. By removing oscillations induced by instrumental or experimental settings, this statistical technique produces the best findings. The created approach was validated using ICH guidelines and found to be simple, linear, accurate, precise, and repeatable. Over a concentration range of 5-15g/mL, the approach was shown to be linear, with a correlation coefficient (r2) of roughly 0.9998. The detection and quantification limits, respectively, were found to be 0.029 and 0.087g/mL. The percentage relative standard deviations for intraday and interday precision were found to be 0.473-1.373 and 0.301-1.617, respectively. The percentage recovery was determined to be between 97.60 and 101.80% w/w.

Conclusion: A simple, linear, precise, accurate, sensitive, and repeatable multivariate calibration technique for the quantification of ivermectin in bulk and pharmaceutical formulation has been devised and verified, according to the findings.

Author(s) Details:

J. Kavitha,
Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kancheepuram, Tamil Nadu, India.


K. S. Lakshmi,
Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kancheepuram, Tamil Nadu, India.

Please see the link here: https://stm.bookpi.org/CAPR-V1/article/view/6233