Because earthworms are vulnerable to predator assaults and agricultural activities, nature has endowed them with the ability to regenerate destroyed body segments. In the lack of a defined nonneural endocrine gland, the cerebral neurosecretory system is likely to play a key role in wound healing and body segment regeneration.
The purpose of this
research is to look at the cytomorphological changes in the cerebral
neurosecretory cells after amputation in the topsoil Indian earthworm Lampito
mauritii.
Dissected cerebral
ganglia were fixed in Bouin's solution and processed for traditional
neurosecretory staining techniques: Paraldehyde Fuchsin (AF) and Chrome Alum
Haematoxylin Phloxin after posterior amputation of the earthworm with a paragon
knife (CAHP).
Following
transection of 10 posteriormost body segments, a wave of muscle contraction was
observed along the anteroposterior direction of Lampito mauritii's body.
Following posterior
amputation of body segments, cerebral neurosecretory cells (especially the AF+ve
A cells) showed changes in secretory dynamics, in contrast to control. Due to
the depletion of neurosecretory resources, the number of AF+ve A cells
decreased during wound healing after 24 hours. The formation of regeneration
blastema was followed by an increase in the number of AF+ve strongly pigmented
A neurosecretory cells 48 to 72 hours after amputation. Coarse secretory
granules were also seen in the moderately stained B cells. After 48 to 72 hours
of amputation, axonal transport and a 'zone of accumulation' charged with AF+ve
neurosecretion were also seen.
Author(S) Details
Anurag Bhattacharjee
Department of Zoology, Tripura University, India.
P. S. Chaudhuri
Department of Zoology, Earthworm Research Laboratory, Tripura University, Suryamaninagar-799022, Tripura, India.
View Book:- https://stm.bookpi.org/NFMMR-V11/article/view/3702
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