Determination of Rilpivirine through LCMS/MS Method: A Novel Approach | Chapter 12 | Novel Aspects on Pharmaceutical Research Vol. 5

 This division highlights a novel approach for Determination of Rilpivirine through LCMS/MS Method. Rilpivirine is a new non-nucleoside reverse transcriptase prevention (NNRTI) that is certified for HIV-1 treatment-naive adult cases in combination accompanying other antiretroviral powers. Over a wide pH range, rilpivirine hydrochloride is basically insoluble in water. The inactive elements croscarmellose sodium, lactose monohydrate, magnesium stearate, polysorbate 20, povidone K30, and silicified microcrystalline and oxygen are also in the direction of each EDURANT tablet. Using a procession Kinetex C18, 100x4.6mm, 2.6µ with a mobile development containing a combination of 0.1% Formic acid and Acetonitrile: Methanol: Isopropanol 65:15:25% v/v in ratio of (5:95) %v/v. The flow rate was 0.6 ml/brief time period and effluent were listened at 282 nm and a peak eluted at 2.06 min and line oven temperature was asserted ambient. Forced shame was used to validate the optimised system for specificity, and it was found that the primary peak remained clean under all degradation income, demonstrating that the approach was a establishment indicating individual.  The method was linear in the range of 0.03 µg/ml to 0.14µg/ml and the equivalence coefficient was erect to be 0.9997. The pattern was also establish to be correct in 50 to 150% of test concentration. The Limit of Quantification of the method was erect to be 10.7836 pg/ml. As the main goal of development of LCMS form is to discover and quantify Rilpivirine at lower concentration, Rilpivirine standard of 0.1PPM were planned from standard stock solution. According to ultimate recent International Conference on Harmonisation (ICH) directions, a mass agreeable liquid chromatographic technique was civilized and validated for specificity, LOD, LOQ, extent of object, accuracy, accuracy, intermediate accuracy, and robustness. The results of the study revealed that the proposed method was erect to be repeatable, exact, linear and correct.

Author(s) Details:

B. Raj Kumar,
Jawaharlal Nehru Technological University Hyderabad, Telangana, India and Faculty of Pharmacy, Kottam Institute of Pharmacy, Erravally X Roads, Jogulamba Gadwal, India.

K. V. Subrahmanyam,
Siddhartha Institute of Pharmacy, Ghatkeskar Mandal, Hyderabad, Telangana, India.

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