The QTLSD7-1/Rc Locus Variation between Weedy (O. sativa f. spontanea) and Wild Rice (O. rufipogon) Populations | Chapter 7 | Research Advances in Microbiology and Biotechnology Vol. 6

 This unit reports on newly discovered DNA sequences that resulted from surveying DNA polymorphism of the QTL/Rc locus in the delegates of wild rice and thin rice accessions geographically originating in Thailand.A pleiotropic position, QTLSD7-1/Rc, regulates edible grain seed dormancy and covering colour. Re-sequencing DNA technology was used to check the promoter region, exon 1, and intron 1 DNA order variation in brown rice from Thailand and Laos, weedy rice accessions from Thailand, in addition to cultivated edible grain. A sample of 54 wild and weedy edible grain accessions including 4 accessions of brown rice and 50 accessions of weedy rice were secondhand in this study. For brown rice, young leaves were collected from mother plants from unrefined habitats of Savannakhet responsibility, Laos (code Rc-OR-LSK1). For promoter domain, three types of DNA variation were noticed including variation in number of nucleotide T, five SNPs and 19-bp indel. For exon 1, no DNA order variation was noticed. For non-coding region intron1, thin rice had the 10-bp insert in the region, but there was the erasure in wild rice. In addition, erasure of 34-bp in intron1 was observed in all brown rice accessions examined, while thin rice had the 34-bp insertion. The QTLSD7-1/Rc is a pleiotropic position of seed abeyance and pericarp color in rice source. Results from this study exhibit DNA sequence difference in weedy rice and brown rice from Thailand and Laos. Haplotype analysis of thin rice indicated that the pairwise nucleotide variety parameter, Pi (𝞹) and the level of the Watterson estimator (⊖w) in the DNA sequences were 0.00401 and 0.0048, individually. Furthermore,  DNA variation show not only as DNA markers in conditions of molecular development, but also weedy edible grain germplasm would be preparation rice toward direct-seeded edible grain could be completed by precisely editing few disadvantaged genes by utilizing the advanced DNA technology.

Author(s) Details:

Preecha Prathepha,
Department of Biotechnology, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham Province-44150, Thailand.

Please see the link here: https://stm.bookpi.org/RAMB-V6/article/view/11161