This chapter demonstrates a rapid, cost-effective, and safe method
for extracting genomic DNA from multiple plant fungal pathogens. Pure cultures
of Fusarium equesti, Neoscytalidium dimidiatum, Fusarium proliferatum, and
Alternaria alternata, isolated from diseased wheat, grapevine, potato, and lily
plants, respectively, were used. The fungal samples were ground with sterilised
sand and 2N NaOH, followed by centrifugation to separate sand grains and fungal
cellular components from the DNA. The resulting DNA was mixed with Tris buffer
(1 M, pH 8). The internal transcribed spacer (ITS) region of ribosomal DNA
(rDNA) was successfully amplified, sequenced, and analysed from the extracted
DNA of all four pathogens. This safe technique offers a straightforward, efficient,
and non-hazardous method to obtain sufficient quantity and quality DNA suitable
for molecular assays to identify plant fungi.
Author(s)
Details
Adnan A. Lahuf
Department of Plant Protection, College of Agriculture, University
of Kerbala, Karbala, Iraq.
Ola H.
Jaafar
Department of Plant Protection, College of Agriculture, University
of Kerbala, Karbala, Iraq.
Zainab
L. Hameed
Department of Field Crops, College of Agriculture, University of
Kerbala, Karbala, Iraq.
Please see the book here:- https://doi.org/10.9734/bpi/crpas/v3/2117
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